Mapping of a novel major effect hessian fly field partial-resistance locus in southern soft red winter wheat line LA03136E71

Authors: WINN, ZACHARY - North Carolina State University; ACHARYA, ROSHAN - North Carolina State University; MERRILL, KEITH - North Carolina State University; LYERLY, JEANETTE - North Carolina State University; Brown-Guedira, Gina; Cambron, Sue; HARRISON, STEPHEN - Louisana State University; REISIG, DOMINIC - North Carolina State University; MURPHY, J PAUL - North Carolina State University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/3/2021
Publication Date: 8/10/2021
Citation: Winn, Z., Acharya, R., Merrill, K., Lyerly, J., Brown Guedira, G.L., Cambron, S.E., Harrison, S., Reisig, D., Murphy, J. 2021. Mapping of a novel major effect hessian fly field partial-resistance locus in southern soft red winter wheat line LA03136E71. Theoretical and Applied Genetics. 134:3911-3923. https://doi.org/10.1007/s00122-021-03936-6.
DOI: https://doi.org/10.1007/s00122-021-03936-6

Interpretive Summary: Hessian flies (Mayetiola destructor Say) infest and feed upon wheat (Triticum aestivum L) resulting in significant yield loss. Genetically resistant cultivars are the most effective method of Hessian fly management. Wheat breeders in the southern USA have observed cultivars exhibiting a “field resistance” to Hessian fly that is not detectable by greenhouse assay. The resistant breeding line “LA03136E71” and susceptible cultivar “Shirley” were crossed to develop a population of 200 random F4:5 lines using single seed descent. The population was evaluated in a total of five locations in North Carolina during the 2019, 2020, and 2021 seasons. A subsample of each plot was evaluated for the total number of tillers, number of infested tillers, and total number of larvae/pupae. From these data, the percent infested tillers, number of larvae/pupae per tiller, and the number of larvae/pupae per infested tiller were estimated. In all within and across environment combinations for all traits recorded, the genotype effect was significant (p < 0.05). Interval mapping identified a single large effect QTL distally on the short arm of chromosome 7D for all environment-trait combinations. This locus was identified on a chromosome where no other Hessian fly resistance/tolerance QTL has been previously identified. This novel Hessian fly partial-resistance QTL is termed QHft.nc-7D. Fine mapping must be conducted in this region to narrow down the causal agents responsible for this trait, and investigation into the mode of action is highly suggested.

Technical Abstract: Hessian flies (Mayetiola destructor Say) infest and feed upon wheat (Triticum aestivum L) resulting in significant yield loss. Genetically resistant cultivars are the most effective method of Hessian fly management. Wheat breeders in the southern USA have observed cultivars exhibiting a “field resistance” to Hessian fly that is not detectable by greenhouse assay. The resistant breeding line “LA03136E71” and susceptible cultivar “Shirley” were crossed to develop a population of 200 random F4:5 lines using single seed descent. The population was evaluated in a total of five locations in North Carolina during the 2019, 2020, and 2021 seasons. A subsample of each plot was evaluated for the total number of tillers, number of infested tillers, and total number of larvae/pupae. From these data, the percent infested tillers, number of larvae/pupae per tiller, and the number of larvae/pupae per infested tiller were estimated. In all within-and-across environment combinations for all traits recorded, the genotype effect was significant (p<0.05). Interval mapping identified a single large effect QTL distally on the short arm of chromosome 7D for all environment-trait combinations. This locus was identified on a chromosome where no other Hessian fly resistance/tolerance QTL have been previously identified. This novel Hessian fly partial-resistance QTL is termed QHft.nc-7D. Fine mapping must be conducted in this region to narrow down the causal agents responsible for this trait and investigation into the mode of action is highly suggested.