Skip to main content
ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #387283
Research Project: Improving Livestock Production by Developing Reproductive and Precision Management Technologies

Location: Livestock Bio-Systems

Title: RNA sequencing and iTRAQ proteomic data from an experiment examining the influence of conceptus presence...preovulatory estradiol on endometrial gene transcripts and proteins..maternal recognition of pregnancy in beef cattle

Author
item NORTHROP-ALBRECHT, EMMALEE - South Dakota State University
item RICH, JERICA - South Dakota State University
item Cushman, Robert - Bob
item YAO, RUNAN - South Dakota State University
item GE, XINJIN - South Dakota State University
item PERRY, GEORGE - South Dakota State University

Submitted to: Data in Brief
Publication Type: Database / Dataset
Publication Acceptance Date: 3/9/2022
Publication Date: 6/1/2022
Citation: Northrop-Albrecht, E.J., Rich, J.J., Cushman, R.A., Yao, R., Ge, X., Perry, G.A. 2022. RNA sequencing and iTRAQ proteomic data from an experiment examining the influence of conceptus presence and preovulatory estradiol on endometrial gene transcripts and proteins around maternal recognition of pregnancy in beef cattle. Data in Brief. 42. Article 108056. https://doi.org/10.1016/j.dib.2022.108056.
DOI: https://doi.org/10.1016/j.dib.2022.108056

Interpretive Summary: Pregnancy failure in beef cattle costs cow-calf producers $600 for a cow that is not pregnant. Maintenance of pregnancy requires complex signaling between the embryo and the uterus. Global analyses of the proteins involved in proper response of the uterus to signals from the embryo were performed. Access to the identity of these proteins is provided to the scientific community to enhance the value of the data and contribute to future hypothesis driven studies or meta-analysis that will dissect the biological roles of these proteins in early embryonic development and successful pregnancies in beef cows.

Technical Abstract: RNA sequencing reads and isobaric tags for a relative and absolute quantification (iTRAQ)-Based Proteomic Data were used to determine the impact of conceptus presence and preovulatory estradiol concentration on function of the d 16 uterus in beef cattle. Conceptuses and endometrial biopsies were collected from the uterine horn ipsilateral to the corpus luteum. Total cellular RNA was extracted from endometrium for RNA sequencing across two lanes of a NovaSeq S2, 2 x 50-bp run. Two independent uterine luminal fluid pools (ULF) were made for each group: highE2/conceptus, highE2/noconceptus, lowE2/conceptus, and lowE2/noconceptus. Peptides were labeled with iTRAQ reagents and analyzed using 2-dimensional liquid chromatography mass spectrometry. Transcript abundances were determined using DESeq2 (FDR <0.05, FC>2). Scaffold Q+ was used to quantitate peptide and protein identifications in ULF. Datasets include uterine transcript and protein abundances among highE2/conceptus vs highE2/noconceptus and lowE2/conceptus vs lowE2/noconceptus groups. This information can be useful for further investigating the role of specific transcripts and proteins in the maintenance of early pregnancy in beef cattle. This dataset is related to the article 'Influence of conceptus presence and preovulatory estradiol exposure on uterine gene transcripts and proteins around maternal recognition of pregnancy in beef cattle' by E.J. Northrop-Albrecht, J.J.J. Rich, R.A. Cushman, R. Yao. X. Ge, G.A. Perry. Molecular and Cellular Endocrinology.