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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #388007
Research Project: Management of Pathogens for Strawberry and Vegetable Production Systems

Location: Crop Improvement and Protection Research

Title: The ancestral chromosome architecture of downy mildew oomycetes revealed by the genome assembly of Peronospora effusa

Author
item FLETCHER, KYLE - University Of California
item SHIN, OON-HA - University Of California
item Clark, Kelley
item FENG, CHUNDA - University Of Arkansas
item PUTMAN, ALEXANDER - University Of California
item CORRELL, JAMES - University Of Arkansas
item Klosterman, Steven
item VAN DEYNZE, ALLEN - University Of California
item MICHELMORE, RICHARD - University Of California

Submitted to: International Congress on Molecular Plant-Microbe Interactions
Publication Type: Abstract Only
Publication Acceptance Date: 9/15/2021
Publication Date: 9/15/2021
Citation: Fletcher, K., Shin, O., Clark, K.J., Feng, C., Putman, A.I., Correll, J.C., Klosterman, S.J., Van Deynze, A., Michelmore, R.W. 2021. The ancestral chromosome architecture of downy mildew oomycetes revealed by the genome assembly of Peronospora effusa. International Congress on Molecular Plant-Microbe Interactions, September 15-16, 2021 (virtual).

Interpretive Summary:

Technical Abstract: Downy mildew of spinach, caused by the oomycete Peronospora effusa, is a disease that occurs worldwide. In this work, the genome of P. effusa isolate UA202013 was assembled using PacBio HiFi reads (32x). Telomere to telomere assemblies of 17 P. effusa chromosomes were generated and sixteen were gapless. The only gap contains the nucleolar organizer region on Chromosome 15. Tight clusters of crinkler effectors were found on larger chromosomes. Dispersed clusters of RxLR effectors were found on smaller chromosomes. Inter-genic distances suggested that the genome was compartmentalized. The genomes of P. effusa and Bremia lactucae share a similar single copy gene order along their 17 chromosomes, though the P. effusa assembly was more repetitive than previously reported. Phylogenetic analysis suggests that the most recent common ancestor of B. lactucae and P. effusa is common to all downy mildews and multiple Phytophthora species, suggesting the 17-chromosome architecture may be ancestral to 100s of oomycete species.