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ARS Home » Southeast Area » Stuttgart, Arkansas » Harry K. Dupree Stuttgart National Aquaculture Research Cntr » Research » Publications at this Location » Publication #388206
Research Project: Enhancing the Production of Hybrid Striped Bass Through Improved Genetics, Nutrition, Production Management, and Fish Health

Location: Harry K. Dupree Stuttgart National Aquaculture Research Cntr

Title: Transcriptomic profiling of white bass (Morone chrysops) under different dietary and temperature rearing conditions

Author
item Fuller, Adam
item Abernathy, Jason
item Bader, Troy
item PEATMAN, ERIC - Auburn University
item Rawles, Steven - Steve
item Webster, Carl

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Database / Dataset
Publication Acceptance Date: 9/28/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: As research continues to explore ways to minimize fishmeal and fish oil in diets for commercial aquaculture, we must continually examine how these alternative formulations impact fish growth and performance. In this regard, optimization of white bass (Morone chrysops) (WB), the female parent of hybrid striped bass (sunshine bass), nutritional needs continues to be explored. The objectives of this study were to evaluate growth, body and tissue composition, and differential gene expression of WB fed one of two diets containing menhaden fish (FS) or flaxseed oil (FX) at two rearing temperatures (22 deg C vs. 26 deg C) for 16 weeks. This research found no difference in final fish weight, VSI, or FCR by either diet (P>0.05). There were significant differences in MR (P=0.005) and HSI (P<0.001) by rearing temperature, but not by diet or diet by temperature (P>0.05). We identified a total of 4910 differentially expressed genes (DEGs) greater than 2-fold in our diet and temperature comparisons, with the majority of these DEGs (99.6%) influenced by differences in diet x temperature interactions. In all treatment comparisons, metabolic process genes and pathways were the most activated, both positively and negatively. For treatment comparisons within the same rearing temperature, the few DEGs identified were involved in glucose and fatty acid metabolism, whereas for diet x temperature comparisons, in addition to metabolic genes, DEGs were identified to be involved in immune response and apoptosis. Higher metabolic activity, apoptosis and immune response within fish reared at 26 deg C was also found when comparing between dietary treatments. Overall, this study indicated that WB reared at the higher temperature were more metabolically active but were also under more physiological and cellular stress. Gene expression data has been submitted to the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and is available to the public under the accession number GSE184557.