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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #388546
Research Project: Genetic Improvement of Biotic and Abiotic Stress Tolerance and Nutritional Quality in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research

Title: Identification of a major QTL for Hessian fly resistance in wheat cultivar ‘Chokwang'

Author
item ZHANG, LIRONG - Kansas State University
item XU, YUNFENG - Kansas State University
item Chen, Ming-Shun
item SU, ZHENQI - China Agricultural University
item LIU, YANG - Kansas State University
item XU, YUZHOU - Kansas State University
item LA, GUIXIAO - Kansas State University
item Bai, Guihua

Submitted to: The Crop Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2021
Publication Date: 8/26/2021
Citation: Zhang, L., Xu, Y., Chen, M., Su, Z., Liu, Y., Xu, Y., La, G., Bai, G. 2021. Identification of a major QTL for Hessian fly resistance in wheat cultivar ‘Chokwang'. The Crop Journal. https://doi.org/10.1016/j.cj.2021.08.004.
DOI: https://doi.org/10.1016/j.cj.2021.08.004

Interpretive Summary: Hessian fly is an important insect pest of wheat that causes stunting and lodging. We evaluated progeny of the cross of cultivars ‘Chokwang’ and ‘Ning 7840’ for Hessian fly resistance and constructed a genetic linkage map using 1,147 DNA markers. One major gene for Hessian fly resistance was identified on chromosome arm 6BS of Chokwang. Four Kompetitive Allele Specific Polymerase Chain Reaction (KASP) markers were developed and validated in a U.S. winter wheat panel. The marker KASP-6B112698 is diagnostic, and can be used to screen for the gene in breeding populations.

Technical Abstract: The Hessian fly (HF, Mayetiola destructor) is one of the destructive pests of wheat (Triticum aestivum L.) worldwide. Resistant cultivars can effectively minimize wheat damage due to this insect pest. To identify new quantitative trait loci (QTL) for HF resistance, a population of recombinant inbred lines (RILs) was developed from a cross between the HF-resistant wheat cultivar ‘Chokwang’ and susceptible wheat ‘Ning 7840’, and phenotyped for responses to HF attack. A linkage map was constructed using 1147 single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS). One major QTL, QHf.hwwg-6BS, for HF-resistance was identified on chromosome arm 6BS, which explained up to 84.0% of the phenotypic variation and was contributed by Chokwang. Two RILs showed recombination in the candidate interval of QHf.hwwg-6BS, which delimited QHf.hwwg-6BS to a 4.75 Mb physical interval between 6,028,601 bp and 10,779,424 bp on chromosome arm 6BS of IWGSC Chinese Spring reference genome RefSeq v2.0. Seven GBS-SNPs in the candidate interval were converted into Kompetitive allele specific polymerase chain reaction (KASP) markers. Two of them, KASP-6B112698 and KASP-6B7901215, were validated in a U.S. winter wheat panel. KASP-6B112698 was nearly diagnostic, thus can be used to screen QHf.hwwg-6BS and pyramid it with other resistance genes in breeding programs.