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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Stored Product Insect and Engineering Research » Research » Publications at this Location » Publication #388853
Research Project: Next-Generation Approaches for Monitoring and Management of Stored Product Insects

Location: Stored Product Insect and Engineering Research

Title: Mobility of phosphine-susceptible and resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) after exposure to controlled release materials with existing…

Author
item RANABHAT, SABITA - Kansas State University
item ZHU, KUN YAN - Kansas State University
item BINGHMA, GEORGINA - University Of Nebraska
item Morrison, William - Rob

Submitted to: Journal of Economic Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/18/2022
Publication Date: 4/16/2022
Citation: Ranabhat, S., Zhu, K., Binghma, G.V., Morrison Iii, W.R. 2022. Mobility of phosphine-susceptible and resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) after exposure to controlled release materials with existing…. Journal of Economic Entomology. 115(3):888-903. https://doi.org/10.1093/jee/toac033.
DOI: https://doi.org/10.1093/jee/toac033

Interpretive Summary: There is interest in developing controlled release materials containing insecticides released over time with novel modes of action to improve resistance management. Our unit has shown that long-lasting insecticide-incorporated netting (LLIN) with deltamethrin has been effectively used against stored-product pests. One aspect that has not been evaluated is how controlled release materials affect phosphine resistant populations at food facilities. We evaluated the efficacy of different controlled release materials (insecticide netting or packaging material) with one of four active ingredients (deltamethrin, permethrin, indoxacarb, and dinotefuran) against phosphine resistant and susceptible lesser grain borer and red flour beetle, and demonstrated that most of the compounds resulted in appreciable and approximately equivalent mortality at higher concentrations regardless of phosphine susceptibility among these two stored product insects. Adults were also exposed for 0.5-60 min and their movement was assessed 0-168 h afterward using video-tracking; exposure to insecticide netting with deltamethrin significantly reduced the movement of both lesser grain borer and red flour beetle compared to the other controlled release materials regardless of their susceptibility to phosphine. Our results demonstrate that controlled release materials can be an additional approach to combat phosphine-resistant populations of stored product insects around food facilities. Other compounds such as permethrin, dinotefuran, and indoxacarb are also effective against phosphine-resistant populations of these key stored product insects except indoxacarb for red flour beetle.

Technical Abstract: BACKGROUND: There is interest in developing controlled release materials (CRMs) with novel modes of action to improve resistance management. Long-lasting insecticide-incorporated netting (LLIN) with deltamethrin has been effectively used against stored-product pests. Here, we evaluated the efficacy of different CRMs (LLIN or packaging material) with each of four active ingredients (AI) (deltamethrin, permethrin, indoxacarb, and dinotefuran) and compared them to control CRMs in reducing movement and increasing mortality of phosphine-susceptible and -resistant Rhyzopertha dominica and Tribolium castaneum. RESULTS: Adults were exposed for 0.5, 2, or 60 min, and their movement was assessed immediately or at 24, or 168 h afterward using video-tracking and Ethovision software. We recorded total distance and velocity traveled by adults. Finally, we tested higher rates of each AI on standardized-sized cheesecloth and varied exposure time to obtain median leathal time (LT50) for each compound and susceptibility. Exposure to LLIN with deltamethrin significantly reduced the movement of both species compared to the other CRMs regardless of their susceptibility to phosphine. Deltamethrin was the most effective AI for both species, while dinotefuran and indoxacarb were the least effective for R. dominica and T. castaneum, respectively. Most AIs resulted in appreciable and approximately equivalent mortality at higher concentrations among phosphine-susceptible and resistant strains. CONCLUSION: Our results demonstrate that CRMs can be an additional approach to combat phosphine-resistant populations of stored product insects around food facilities. Other compounds such as permethrin, dinotefuran, and indoxacarb are also effective against phosphine-resistant populations of these key stored product insects except indoxacarb for T. castaneum.