Jasmonates promote enhanced production of bioactive caffeoylquinic acid derivative in Eclipta prostrata (L.) L. hairy roots

Authors: MACIEL, GEVERALDO - Universidade De Ribeirao Preto; APARECIDA LOPES, ADRIANA - Universidade De Ribeirao Preto; Cantrell, Charles; DE CASTRO FRANCA, SUZELEI - Universidade De Ribeirao Preto; WALERIA BERTONI, BIANCA - Universidade De Ribeirao Preto; VERGINIA LOURENCO, MIRIAM - Universidade De Ribeirao Preto

Submitted to: Plant Cell Tissue and Organ Culture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/16/2021
Publication Date: 11/22/2021
Citation: Maciel, G., Aparecida Lopes, A., Cantrell, C.L., De Castro Franca, S., Waleria Bertoni, B., Verginia Lourenco, M. 2021. Jasmonates promote enhanced production of bioactive caffeoylquinic acid derivative in Eclipta prostrata (L.) L. hairy roots. Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240-021-02201-4.
DOI: https://doi.org/10.1007/s11240-021-02201-4

Interpretive Summary: In vitro culture techniques are an alternative for the rapid multiplication of rare plant genotypes, plant genome transformation, and production of plant bioactives, they allow the use of biotechniques that may increase plant secondary metabolite production. Applications of some elicitors and combinations of different stress stimuli can enhance the production of bioactive molecules by elicitor effects. Jasmonates are considered widespread elicitors since they affect several signaling pathways stimulating the production of different classes of secondary metabolites in plants and microorganisms. Eclipta alba is widely used in traditional medicine against several diseases like hepatitis, poisoning by snake bites and viral infections and pharmacological studies confirmed its antioxidant, anti-inflammatory and anticancer activities. The efficacy of E. alba extracts have been correlated to phenylpropanoids such as flavonoids, coumestans and caffeoylquinic acid derivatives. In this work the production of three important phytochemicals in hairy root cultures of Eclipta alba C19 clone was increased after addition of eliciting agents jasmonic acid (JA) or methyl jasmonate (MeJA) at multiple concentrations. Obtained data validate the potential of E. alba hairy root cultures as a production system for these compounds.

Technical Abstract: Eclipta alba is widely used in traditional medicine against several diseases like hepatitis, poisoning by snake bites and viral infections and pharmacological studies confirmed its antioxidant, anti-inflammatory and anticancer activities. The efficacy of E. alba extracts have been correlated to phenylpropanoids such as flavonoids, coumestans and caffeoylquinic acid derivatives. In this work the production of wedelolactone, demethylwedelolactone and 3,5-di-O-caffeoylquinic acid (3,5-diCQA) in hairy root cultures of Eclipta alba C19 clone was increased after addition of eliciting agents jasmonic acid (JA) or methyl jasmonate (MeJA) at multiple concentrations. Cultures elicited with 100 µM of JA induced 5.2 fold wedelolactone (from 0.72 mg/g dw to 3.72 mg/g dw), 1.6 fold demethylwedelolactone (from 5.54 mg/g dw to 9.04 mg/g dw) and 2.47 fold 3,5-di-O-caffeoylquinic acid (from 18.08 mg/g dw to 44.71 mg/g dw). Obtained data validate the potential of E. alba hairy root cultures as a production system of wedelolactone, demethylwedelolactone and specifically 3,5-di-O-caffeoylquinic acid.