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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #389898
Research Project: Management, Characterization, and Evaluation of Pacific Tropical and Subtropical Fruit and Nut Genetic Resources and Associated Information

Location: Tropical Plant Genetic Resources and Disease Research

Title: Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus

Author
item LARREA-SARMIENTO, ANDRIANA - University Of Hawaii
item GEERING, ANDREW - University Of Queensland
item OLMEDO-VELARDE, ALEJANDRO - University Of Hawaii
item WANG, XUPENG - University Of Hawaii
item BORTH, WAYNE - University Of Hawaii
item Matsumoto Brower, Tracie
item Suzuki, Jon
item Wall, Marisa
item MELZER, MICHAEL - University Of Hawaii
item MOYLE, RICHARD - University Of Queensland
item SHARMAN, MURRAY - Department Of Agriculture And Fisheries
item HU, JOHN - University Of Hawaii
item THOMAS, JOHN - University Of Queensland

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/28/2022
Publication Date: 10/21/2022
Citation: Larrea-Sarmiento, A., Geering, A., Olmedo-Velarde, A., Wang, X., Borth, W., Matsumoto Brower, T.K., Suzuki, J.Y., Wall, M.M., Melzer, M., Moyle, R., Sharman, M., Hu, J., Thomas, J. 2022. Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus. Archives of Virology. 167:2801-2804. https://doi.org/10.1007/s00705-022-05590-9.
DOI: https://doi.org/10.1007/s00705-022-05590-9

Interpretive Summary: A novel virus in pineapple was discovered by high-throughput sequencing in plantsshowing symptoms of Mealybug Wilt. This virus consists of two parts, the first contained five domains associated with the replication of the virus and the second part contained the proteins to protect the virus and assist with movement within the plant. Comparison of the sequence revealed, this virus is similar to pineapple secovirus A, chocolate lily virus A, and Dioscorea mosaic-associated virus, thus will be called pineapple secovirus B.

Technical Abstract: The complete genome sequence of a member of a novel species of Sadwavirus (family Secoviridae) infecting pineapple on the island of Oahu, Hawaii, was determined by high throughput sequencing. The genome comprised two RNA molecules of 5,958 nt and 3,922 nt in length, excluding the poly-A tails at the 3’ end and each encoding a single large polyprotein. The RNA-1 polyprotein contained five conserved domains, all associated with replication, while the RNA-2 polyprotein is cleaved into the movement protein and coat protein. Analysis of the Pro-Pol region revealed less than 75% amino acid identity with pineapple secovirus A, chocolate lily virus A, and Dioscorea mosaic-associated virus, all members and candidate members of the proposed subgenus Cholivirus. Two specific primer sets derived from HTS data were used in RT-PCR assays, confirming the presence of this new virus infecting pineapple. The name “pineapple secovirus B” is proposed for this putative new virus.