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Research Project: Exotic Whitefly and Subtropical Invasive Pests of Vegetables and Ornamental Plants

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Title: Pragmatic applications and universality of DNA barcoding for substantial organisms at species level: A review to explore a way forward

Author
item AHMED, SARFRAZ - University Of Veterinary And Animal Sciences
item IBRAHIM, MUHAMMAD - Bahauddin Zakariya University
item NANTASENAMAT, CHANIN - Mahidol University
item NISAR, MUHAMMAD FARRUK - University Of Veterinary And Animal Sciences
item MALIK, AIJAZ AHMAD - Mahidol University
item WAHEED, RASHEM - Bahauddin Zakariya University
item Ahmed, Muhammad
item ALAM, MOHAMMAD KHURSH - Jouf University
item OJHA, SUVASH CHANDRA - Luzhou Medical College

Submitted to: BioMed Research International
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/6/2021
Publication Date: 1/11/2022
Citation: Ahmed, S., Ibrahim, M., Nantasenamat, C., Nisar, M.H., Malik, A., Waheed, R., Ahmed, M.Z., Alam, M.E., Ojha, S. 2022. Pragmatic applications and universality of DNA barcoding for substantial organisms at species level: A review to explore a way forward. BioMed Research International. 2022:1846485. https://doi.org/10.1155/2022/1846485.
DOI: https://doi.org/10.1155/2022/1846485

Interpretive Summary: DNA barcoding has numerous inventive traits that have undertaken continuous improvements in its wide array of applications in life science. DNA barcoding in different organisms ranging from unicellular to multicellular is not yet 100% reliable. Future efforts should be made to develop nuclear barcodes to augment DNA barcodes. The DNA barcodes are at the beginning of their application in species discovery but can turn into a standard identification protocol for all living organisms.

Technical Abstract: DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome oxidase 1 (CO1) gene as a DNA barcode is highly efficient for discriminating vertebrate and invertebrate animal species. Similarly, different specific markers are used for other organisms, including ribulose bisphosphate carboxylase (rbcL), maturase kinase (matK), transfer RNA-H and photosystem II D1-ApbsArabidopsis thaliana (trnH-psbA), and internal transcribed spacer (ITS) for plant species; 16s ribosomal RNA (16S rRNA), elongation factor Tu gene (Tuf gene) and chaperonin for bacterial strains; and nuclear ITS for fungal strains. Nevertheless, the taxon coverage of reference sequences is far from complete for genus or species-level identification. Applying the next-generation sequencing approach to the parallel acquisition of DNA barcode sequences could greatly expand the potential for library preparation or accurate identification in biodiversity research. Overall, this review articulates on the DNA barcoding technology as applied to different organisms, its universality, applicability and innovative approach to handling DNA-based species identification.