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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #390531
Research Project: Genetic Improvement and Sustainable Production Systems for Sub-tropical and Tropical Crops in the Pacific Basin

Location: Tropical Plant Genetic Resources and Disease Research

Title: Complete genome organization and characterization of Hippeastrum latent virus

Author
item WANG, XUPENG - University Of Hawaii
item LARREA-SARMIENTO, ANDRIANA - University Of Hawaii
item OLMEDO-VELARDE, ALEJANDRO - University Of Hawaii
item BORTH, WAYNE - University Of Hawaii
item Suzuki, Jon
item Wall, Marisa
item MELZER, MICHAEL - University Of Hawaii
item HU, JOHN - University Of Hawaii

Submitted to: Virus Genes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/1/2022
Publication Date: 4/15/2022
Citation: Wang, X., Larrea-Sarmiento, A., Olmedo-Velarde, A., Borth, W., Suzuki, J.Y., Wall, M.M., Melzer, M., Hu, J. 2022. Complete genome organization and characterization of Hippeastrum latent virus. Virus Genes. 58:367-371. https://doi.org/10.1007/s11262-022-01901-z.
DOI: https://doi.org/10.1007/s11262-022-01901-z

Interpretive Summary: High throughput sequencing was used to determine the possible presence of plant viruses correlated with ringspot and mosaic symptoms on leaves of spider lily, a commonly grown ornamental in Hawaii. Results identify two closely related, but distinct plant carlaviruses from these diseased plants including the first characterization of the entire Hippeastrum latent virus (HiLV) genome. This is the first report of plant HiLV in spider lily and in the U.S.

Technical Abstract: The complete genome sequences of two carlaviruses were determined by high-throughput sequencing of RNA extracted from ringspot and mosaic, disease symptoms on leaves of spider lily plants (Crinum asiaticum, family Amaryllidaceae) growing as landscape plants in Hawaii. One, named Nerine latent virus (NeLV)-Hawaii with a genome of 8281 nucleotide exhibited the highest nucleotide identity and amino acid similarity of 95.5% and 96.0%, respectively, to the genome sequence of an isolate of NeLV from Narcissus sp. in Australia (JQ395044). The second, named Hippeastrum latent virus (HiLV)-Hawaii with a genome of 8497 nucleotides exhibited the highest nucleotide identity and amino acid similarity, 84.3% and 88.7%, respectively, to the sequence of a previously uncharacterized HiLV isolate from a potted fowering plant, Amaryllis (Hippeastrum hybridum Hort) in Taiwan (DQ098905). The amino acid sequence similarities of replicase (Rep) and coat protein (CP) between HiLV-Hawaii and NeLV-Hawaii were 44.8% and 38.4%, respectively. Results of viral protein Rep and CP amino acid sequence comparisons from various carlaviruses provide evidence that HiLV and NeLV, previously classifed as synonymous viruses are in fact unique viruses. This is the frst report for the complete sequence, organization, and phylogenetic characterization of HiLV and the frst detection of HiLV both in C. asiaticum and in the USA.