Skip to main content
ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #390572
Research Project: Potato Genetic Improvement for Enhanced Tuber Quality and Greater Productivity and Sustainability in Western U.S. Production

Location: Small Grains and Potato Germplasm Research

Title: A potential new source of extreme resistance to Potato virus Y in the potato variety Bistra

Author
item ELISON, GREGORY - Cibus
item PARK, JAEBUM - Orise Fellow
item Novy, Richard - Rich
item Whitworth, Jonathan

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/15/2024
Publication Date: 4/26/2024
Citation: Elison, G.L., Park, J., Novy, R.G., Whitworth, J.L. 2024. A potential new source of extreme resistance to Potato virus Y in the potato variety Bistra. American Journal of Potato Research. 101:248-256. https://doi.org/10.1007/s12230-024-09954-6.
DOI: https://doi.org/10.1007/s12230-024-09954-6

Interpretive Summary: Potato virus Y (PVY) is a devastating disease which reduces potato yield and tuber quality. Genetic resistance to PVY is conferred by three genes which can be bred into domestic potato varieties and completely prevent infection by the virus with the use of marker assisted selection (MAS). However, many varieties exist which have resistance to PVY, but do not amplify any markers used in MAS, making them difficult to use to breed for resistance to the virus. Sante and its descendent Bistra are two such varieties utilized in the Aberdeen breeding program. In an attempt to develop markers for Sante and Bistra, 190 descendents of a cross between Bistra and a susceptible cultivar were mapped to determine the genomic location of the PVY resistance gene found in Bistra. The gene was found on one end of chromosome 11 in close proximity to a known source of PVY resistance. However, none of the genetic markers for this other source amplified in Bistra, indicating the likely discovery of a new source of PVY resistance in Bistra. This is the first step in developing markers for the resistance found in Bistra which would allow its use to breed for PVY resistance in the Aberdeen breeding program.

Technical Abstract: Potato virus Y (PVY) is a common and devastating pathogen of potato worldwide and insecticide control alone for aphid vectors is not effective due to the rapid transmission of the virus by aphids following probing. Three Ry genes for extreme resistance to PVY have been identified and are used in potato breeding programs for the development of PVY-resistant varieties. Tightly linked molecular markers have been developed for all three genes and are used for marker-assisted selection (MAS). However, some varieties with known extreme resistance to PVY do not amplify any molecular markers for these resistance genes. Sante and its progeny Bistra are examples of two varieties with extreme resistance to PVY and yet have no amplification of Ry associated molecular markers. The source of PVY resistance in Bistra was mapped using SNP genotyping of 190 of its progeny which had been characterized for response to PVY infection using a N-Wilga strain. A major QTL was found on the proximal end of chromosome 11, in close proximity to Ryadg, one of the three known extreme resistance genes for PVY. However, no known markers which detect Ryadg amplify in Sante or Bistra which indicates they may possess a currently unidentified resistance gene, or the genetic linkages of molecular markers typically associated with the presence of Ryadg no longer exist due to recombination between the gene and its associated molecular marker primer annealing sites.