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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Environmental Microbial & Food Safety Laboratory » Research » Publications at this Location » Publication #391088
Research Project: Intervention Strategies to Mitigate the Food Safety Risks Associated with the Fresh Produce Supply Chain

Location: Environmental Microbial & Food Safety Laboratory

Title: Rapid detection of Salmonella Enterica in fresh produce by a novel Microarray-based PathogenDx system

Author
item Yin, Hsin Bai
item CHEN, CHI-HUNG - Orise Fellow
item KATCHMAN, BENJAMIN - Pathogendx
item NEWLAND, CORY - Pathogendx
item TOMCHANEY, MICHAEL - Pathogendx
item ULRICHM, PEACHES - Pathogendx
item FREEMAN, SHAYLA - Pathogendx
item MAY, MELISSA - Pathogendx
item EGGERS, RICK - Pathogendx
item Patel, Jitu
item O'BRIEN, KEVIN - Pathogendx
item HOGAN, MICHAEL - Pathogendx

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: 3/21/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Introduction: Consumption of fresh produce contaminated with Salmonella enterica may result in significant risk of foodborne illnesses. The diverse matrices pose great challenges for rapid Salmonella detection. Purpose: The efficacy of PathogenDx microarray-based detection system for the rapid detection of Salmonella in fresh produce was investigated. Methods: A pre-PCR sample preparation protocol including enrichment in universal pre-enrichment broth for 3 h with a sample concentration step by using a concentrator or 6 h enrichment without a concentration step was used for detecting Salmonella enterica Newport (~ 6 CFU/25 g) from kale, spinach, Romaine lettuce, and Iceberg lettuce (N=205). Samples were simultaneously analyzed by the FDA-BAM Salmonella detection procedure. Results: The system correctly identified all 108 Salmonella strains and 35 non-Salmonella strains. Among 205 produce samples tested, 98%, 93%, 76%, and 60% of Romaine lettuce, Iceberg lettuce, kale, and spinach samples were detected after 3 h of enrichment with sample concentration. After 6 h of enrichment, 100%, 98%, 90%, and 82% of Romaine lettuce, Iceberg lettuce, kale, and spinach samples were successfully detected. The overall analysis time of this methodology was between 8-11 h, in contrast to 4 to 5 days of analysis time for the traditional culture method. Significance: The DetectX Food DNA microarrays allow for the rapid detection of multiple pathogens from a single sample with minimal enrichment, providing results in a single shift. Here we have demonstrated the utility of this assay for the rapid detection of Salmonella in fresh produce.