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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #391425
Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: A genome resource for Xylella fastidiosa subspecies multiplex strain P5A2 causing phony peach disease in the southeastern United States

Author
item JOHNSON, K.A - University Of Georgia
item Bock, Clive
item BRANNEN, P.M. - University Of Georgia
item Chen, Jianchi

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/6/2022
Publication Date: 11/19/2022
Citation: Johnson, K., Bock, C.H., Brannen, P., Chen, J. 2022. A genome resource for Xylella fastidiosa subspecies multiplex strain P5A2 causing phony peach disease in the southeastern United States. Phytopathology. 122(11):2466-2470. https://doi.org/10.1094/PHYTO-03-22-0081-A.
DOI: https://doi.org/10.1094/PHYTO-03-22-0081-A

Interpretive Summary: Phony peach disease (PPD) causes yield loss and orchard decline of peach in the southeastern U.S. PPD is caused by a nutritionally fastidious bacterium, Xylella fastidiosa subsp. multiplex (Xfm), which is difficult to culture in artificial media. Biological information about the PPD bacteria is limited. Using a recently developed biotechnology tool, called metagenomics, we by-passed the difficulty of in vitro culture and obtained the first whole genome sequence of a PPD bacterium in the southeastern U.S. The genome sequence will provide a valuable resource for studies of the population biology, pathogenicity, and management of PPD.

Technical Abstract: Phony peach disease (PPD) is caused by a nutritionally fastidious bacterium, Xylella fastidiosa subsp. multiplex (Xfm), resulting in yield loss and orchard decline of peach (Prunus persica) in the southeastern U.S. We report the first draft whole genome sequence of Xfm strain P5A2 (2,461,230 bp) accompanied by a plasmid (27,734 bp) from a PPD affected peach tree in Alabama using a metagenomic approach that by-passed the process of in vitro culture. The genome sequence will provide a valuable resource for studies of the population biology, pathogenicity, and management of PPD.