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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #391806
Research Project: Development of Novel Cottonseed Products and Processes

Location: Commodity Utilization Research

Title: Bacterial endotoxin lipopolysaccharides regulate gene expression in human colon cancer cells

Author
item Cao, Heping

Submitted to: BMC Research Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/6/2023
Publication Date: 9/13/2023
Citation: Cao, H. 2023. Bacterial endotoxin lipopolysaccharides regulate gene expression in human colon cancer cells. BMC Research Notes. 16. Article 216. https://doi.org/10.1186/s13104-023-06506-9.
DOI: https://doi.org/10.1186/s13104-023-06506-9

Interpretive Summary: Colon cancer is one of the deadliest diseases in the World. The lifetime risk of developing colorectal cancer is approximately 4.0% in 2021. It is urgently needed to fully understand the mechanism of developing colon cancer and explore ways to ease the burden of the healthcare crisis. Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. LPS is a bacterial endotoxin proposed to have antitumor effect. Previous studies have explored the effects of LPS on gene expression in colon cancer cells, but they were focused on very few targets. The objective of this study was to survey the effects of LPS on cell viability and gene expression of 55 molecular targets in human colon cancer cells. We observed that LPS did not affect the viability of COLO 225 cells under the culture conditions but affected the expression of a number of genes important in inflammatory responses and cancer development. These data suggest that LPS has profound effects on gene expression and should provide a basis for mechanistic study of LPS on the regulation of gene expression in human colon cancer cells.

Technical Abstract: Objective: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. Colon bacteria contribute to LPS which promotes colon cancer metastasis. The objective of this study was to survey the effect of LPS on cell viability and gene expression of 55 molecular targets in human colon cancer cells. Results: LPS did not affect the viability of COLO 225 cells under the culture conditions but affected the expression of a number of genes important in inflammatory responses and cancer development. LPS increased TTP family, GLUT family and DGAT1 mRNA levels but decreased DGAT2a and DGAT2b expression in the human colon cancer cells. LPS also increased COX2, CXCL1, ELK1, ICAM1, TNFSF10 and ZFAND5 but decreased BCL2L1, CYP19A1 and E2F1 mRNA levels in the colon cancer cells. These data suggest that LPS has profound effects on gene expression in human colon cancer cells.