Bacterial endotoxin lipopolysaccharides regulate gene expression in human colon cancer cells

Authors: Cao, Heping

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/26/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. Colon bacteria contribute to LPS which promotes colon cancer metastasis by enhancing tumor cell adhesion, intravasation, and extravasation. Previous studies explored the effects of LPS on gene expression in colon cancer cells, but they were focused on very few targets. The objective of this study was to survey the effects of LPS on cell viability and gene expression of 55 molecular targets in human colon cancer cells. The author observed that LPS did not affect the viability of COLO 225 cells under the culture conditions but affected the expression of a number of genes important in inflammatory responses and cancer development. LPS increased TTP family, GLUT family and DGAT1 mRNA levels but decreased DGAT2a and DGAT2b expression in the human colon cancer cells. LPS also increased COX2, CXCL1, ELK1, ICAM1, TNFSF10 and ZFAND5 but decreased BCL2L1, CYP19A1 and E2F1 mRNA levels in the colon cancer cells. LPS did not exhibit significant effects on the mRNA levels of BNIP3, FAS, HUA, ILs, HUA, LEPTIN P53, PPARR and TNF genes in the human colon cancer cells. These data suggest that LPS has profound effects on gene expression and should provide a basis for mechanistic study of LPS on the regulation of gene expression in human colon cancer cells.