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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #392804
Research Project: Improve Nutrient Management and Efficiency of Beef Cattle and Swine

Location: Nutrition, Growth and Physiology

Title: One-carbon metabolite supplementation influences pancreatic a-amylase activity and protein concentration in a dose-dependent manner in beef heifers

Author
item TROTTA, RONALD - University Of Kentucky
item Freetly, Harvey
item Cushman, Robert - Bob
item Oliver, William
item Lindholm-Perry, Amanda
item CATON, JOEL - North Dakota State University
item WARD, A - North Dakota State University
item Crouse, Matthew

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2022
Publication Date: 9/21/2022
Citation: Trotta, R.J., Freetly, H., Cushman, R.A., Oliver, W., Lindholm-Perry, A., Caton, J.S., Ward, A.K., Crouse, M.S. 2022. One-carbon metabolite supplementation influences pancreatic a-amylase activity and protein concentration in a dose-dependent manner in beef heifers [abstract]. Journal of Animal Science. 100(Supplement 3):259. https://doi.org/10.1093/jas/skac247.469.
DOI: https://doi.org/10.1093/jas/skac247.469

Interpretive Summary:

Technical Abstract: The objective of this study was to evaluate different combinations of one-carbon metabolites [OCM: rumen-protected methionine (RPM), rumen-protected choline (RPC), folate (FA), vitamin B12 (B12)] on pancreatic and jejunal carbohydrase activity at d 14 of the estrous cycle in beef heifers. Thirty Angus heifers were fed a high-roughage diet and assigned to treatments: 0% RPM + 0 g/d RPC + saline injection (0XNEG), 0.08% RPM + 60 g/d RPC + saline injection (0XPOS), 0.08% RPM + 60 g/d RPC + 80 mg FA + 5 mg B12 (0.5X), 0.08% RPM + 60 g/d RPC + 160 mg FA + 10 mg B12 (1X), or 0.08% RPM + 60 g/d RPC + 320 mg FA + 20 mg B12 (2X). Dietary treatments were fed for 14 d and FA and B12 injections were given on d 0 and 7 of the estrous cycle with tissues collected on d 14. The effect of RPM+RPC supplementation was evaluated using a contrast statement comparing 0XNEG vs. others. Polynomial contrasts were used to determine effects of FA+B12 dose. Protein concentration and carbohydrase activities of the pancreas and jejunum were not influenced (P=0.21) by RPM+RPC supplementation. Pancreatic protein concentration responded cubically (P< 0.01) to FA+B12 dose where pancreatic protein concentration was lesser for 0.5X compared with other treatments. Likewise, pancreatic a-amylase activity responded cubically (P< 0.01) to increasing FA+B12 dose because pancreatic a-amylase activity was lesser for 0.5X (182 U/g) compared with 0XPOS (350 U/g), 1X (347 U/g), and 2X (412 U/g). Jejunal protein concentration and maltase and glucoamylase activities were not influenced (P=0.29) by FA+B12 dose. Jejunal isomaltase activity tended to decrease linearly (P=0.07) with increasing FA+B12 dose. These findings suggest that the balance of different OCM could be important for regulation of pancreatic protein turnover and a-amylase activity.