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ARS Home » Northeast Area » Frederick, Maryland » Foreign Disease-Weed Science Research » Research » Publications at this Location » Publication #392827
Research Project: Molecular Identification, Characterization, and Biology of Foreign and Emerging Viral and Bacterial Plant Pathogens

Location: Foreign Disease-Weed Science Research

Title: Maize rayado fino virus coat proteins: CP2 is required for systemic infection and CP1 is required for leafhopper transmission

Author
item MLOTSHWA, SIZOLWENKOSI - The Ohio State University
item KHATRI, NITIKA - The Ohio State University
item WILLIE, KRISTEN - US Department Of Agriculture (USDA)
item XU, JUNHUAN - The Ohio State University
item TODD, JANE - US Department Of Agriculture (USDA)
item TRAN, HONG HAHN - The Ohio State University
item Stewart, Lucy

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2022
Publication Date: 6/24/2023
Citation: Mlotshwa, S., Khatri, N., Willie, K., Xu, J., Todd, J., Tran, H., Stewart, L.R. 2023. Maize rayado fino virus coat proteins: CP2 is required for systemic infection and CP1 is required for leafhopper transmission. American Phytopathological Society Annual Meeting. 112:S3.1. https://doi.org/10.1094/PHYTO-112-11-S3.1.
DOI: https://doi.org/10.1094/PHYTO-112-11-S3.1

Interpretive Summary:

Technical Abstract: Maize rayado fino virus (MRFV), like other marafiviruses, encodes two carboxy co-terminal coat proteins, CP1 and CP2. Using a series of mutants, we examined the expression of each coat protein either from a subgenomic RNA predicted to be driven by a marafibox promoter, or by proteolytic cleavage of the polyprotein expected to release a fraction of CP1. Expression of MRFV CP1 by polyprotein release was minimal. CP1 mutants resulted in leaky, low-level expression of CP1 and showed systemic infection and symptoms in plants, but lost leafhopper transmissibility. Mutants impacting CP2 expression resulted in no systemic infection of plants. Together, results indicated that CP2 is required to establish systemic infection, while CP1 is required for leafhopper transmission.