Proteomic dataset comparing strains of Leptospira borgpetersenii serovar Hardjo cultured at different temperatures

Authors: Putz, Ellie; FERNANDES, LUIS - Butantan Institute; Bayles, Darrell; Lippolis, John; Nally, Jarlath

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/25/2022
Publication Date: 12/1/2022
Citation: Putz, E.J., Fernandes, L.G., Bayles, D.O., Lippolis, J.D., Nally, J.E. 2022. Proteomic dataset comparing strains of Leptospira borgpetersenii serovar Hardjo cultured at different temperatures. Data in Brief. 45. https://doi.org/10.1016/j.dib.2022.108713.
DOI: https://doi.org/10.1016/j.dib.2022.108713

Interpretive Summary: Leptospira interrogans is the leading cause of human leptospirosis and L. borgpetersenii is a leading cause of animal leptospirosis. Compared to L. interrogans, few studies have defined the proteome composition of L. borgpetersenii, an essential requirement for the development of efficacious animal vaccines. The leading cause of bovine leptospirosis is Leptospira borgpetersenii serovar Hardjo. In this study, we defined and compared the proteome of two recent isolates of L. borgpetersenii serovar Hardjo and compared them to the proteome of two bovine isolates obtained over 30 years ago. This work facilitates continued comparative proteome analysis with other serovar Hardjo isolates, separated over time and geography, to identify conserved protein features, understand evolutionary traits that predispose serovar Hardjo to persistent carriage in cattle and zoonotic transmission, and gain additional insights into pathogenic mechanisms of infection.

Technical Abstract: Leptospirosis is a global zoonotic bacterial disease which is a threat for humans and most mammals. Bacterin vaccines for leptospirosis are available however they are severely limited in cross protection between serogroups. Leptospira typically colonize the kidneys of reservoir hosts where they are subsequently shed in the urine and persist in the environment and can thus be indirectly or directly transmitted to incidental hosts. Leptospira borgpetersenii serovar Hardjo is the primary cause of leptospirosis in cattle which can results in abortion, unhealthy calves, and rebreed problems. This dataset comprises proteomic profiles of four strains of L. borgpetersenii serovar Hardjo propagated at the routinely utilized culture temperature of 29°C, and a newly achieved culture temperature of 37°C, which more closely emulates the temperature of an infected host. The strains analyzed include JB197 (established strain that causes Hardjo atypical acute disease in the hamster model of leptospirosis), HB203 (established strain, causes typical chronic disease in hamsters), as well as TC129 and TC273 (recently isolated strains from the central United States). Differential expression profiles were detected not only between strains but also within strains between culture temperatures. Mass spectrometry data are available via ProteomeXchange with identifier PXD032831.