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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #393424
Research Project: Improve Nutrient Management and Efficiency of Beef Cattle and Swine

Location: Nutrition, Growth and Physiology

Title: The effect of one-carbon metabolite supplementation in combination with plane of nutrition during early gestation on maternal serum and fetal fluids

Author
item ENTZIE, Y - North Dakota State University
item KING, L - North Dakota State University
item SYRING, J - North Dakota State University
item HIRCHERT, M - North Dakota State University
item CATON, J - North Dakota State University
item Crouse, Matthew
item DAHLEN, C - North Dakota State University
item WARD, A - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/22/2022
Publication Date: 8/28/2022
Citation: Entzie, Y.L., King, L.E., Syring, J.G., Hirchert, M., Caton, J.S., Crouse, M.S., Dahlen, C.R., Ward, A.K. 2022. The effect of one-carbon metabolite supplementation in combination with plane of nutrition during early gestation on maternal serum and fetal fluids [abstract]. 2022 Aspen Perinatal Biology Symposium. Abstract I-XIV (Scientific Program p. 28). Available: https://www.asas.org/meetings/perinatal-biology-symposium-2022/scientific-program-(draft)

Interpretive Summary:

Technical Abstract: Objectives: Our objective was to evaluate the effect of one-carbon metabolites (OCM; folate, vitamin B12, choline, and methionine) supplementation in combination with plane of nutrition in early gestation on maternal serum glucose, BUN, and NEFA concentrations as well as fructose and glucose concentrations in allantoic and amniotic fluid. Methods: Thirty-two cross-bred Angus heifers were estrous synchronized and artificially inseminated with female-sexed semen. At breeding (day 0), heifers were assigned to treatments in a 2 × 2 factorial design. The first factor was gain: control (CON; 0.60 kg/day ADG) versus restricted (RES; -0.23 kg/day). The second factor was OCM: supplementation (+OCM; ruminal protected choline [0.60 g/day] and methionine [20 g/day] in a ground corn carrier, and weekly injections of 320 mg folate and 20mg vitamin B12) or no supplementation (-OCM; corn carrier and saline injections). Blood samples were collected on day 0, 35 and 62. Allantoic and amniotic fluid were collected on day 63 relative to breeding. Data were analyzed using the MIXED procedure of SAS with day as repeated measure for glucose, BUN, and NEFA with significance at P < 0.05. Results: Serum glucose was greater in CON than RES (3.44 vs 3.21 ± 0.07 mM; P = 0.03). The gain by day interaction was significant for BUN and NEFA (P < 0.05), with BUN concentrations increasing and NEFA decreasing over time for CON but not for RES. Amniotic fructose concentration was greater in CON-OCM than CON+OCM and RES-OCM, with RES+OCM intermediate (P = 0.005). Allantoic glucose concentration tended to be greater (P = 0.09) for CON-OCM than CON+OCM. Conclusions: We conclude that supplementation of OCM in early gestation increases concentrations of energy metabolites glucose and fructose in fetal fluids of RES heifers. (Supported by USDA-NIFA-AFRI 2018-07055).