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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #393567
Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Practical applications of disinfectants to manage Tomato brown rugose fruit virus in greenhouse tomatoes

Author
item Ling, Kai-Shu
item Gilliard, Andrea

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2022
Publication Date: 11/1/2022
Citation: Ling, K., Gilliard, A.C. 2022. Practical applications of disinfectants to manage Tomato brown rugose fruit virus in greenhouse tomatoes. Phytopathology. 112:S3.141. https://doi.org/10.1094/PHYTO-112-11-S3.1.
DOI: https://doi.org/10.1094/PHYTO-112-11-S3.1

Interpretive Summary: N/A

Technical Abstract: Our previous efforts in screening disinfectants have identified several chemicals that are effective in deactivating virus infectivity to two common tobamoviruses: tomato brown rugose fruit virus (ToBRFV) and cucumber green mottle mosaic virus (CGMMV). However, for practical applications of the disinfectants to manage emerging ToBRFV, some improvements would be necessary to optimize the concentration and potential phytotoxicity of disinfectants on treated tomato plants. We were also interested in evaluating whether a systemic acquired resistance (SAR) from SP2700/Ninja (SePRO, Carmel, IN) would improve the efficacy of disinfectants against ToBRFV in tomato plants. Results showed that although symptom expression was delayed for 1-3 weeks on tomato plants treated with SP2700 in comparison to control plants (no SP2700 treatment), the SAR did not generate sufficient immunity against ToBRFV infection. On disinfection, using 11 chemicals at 3 concentrations for treatment against ToBRFV, 2% Virkon S, 5% Clorox bleach solution and 2.5% trisodium phosphate (TSP) achieved complete deactivation of ToBRFV. Virocid at 0.5% had only partial effect (50%), although our earlier study had a near complete efficacy to ToBRFV. To achieve better assessment on Virocid, a serial dilution of Virocid (0.5% to 2%) was used. Although the efficacy against ToBRFV from 0.5% and 0.75% had inconsistent effect, those higher concentration (>1%) achieved full protection. These evaluations offered practical solutions to select the most effective disinfectants to manage emerging ToBRFV in greenhouse tomatoes.