Skip to main content
ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #393569
Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Effectiveness of disinfectants to manage the emerging tomato brown rugose fruit virus in greenhouse tomato

Author
item Abrahamian, Peter
item CAI, WEILI - Animal And Plant Health Inspection Service (APHIS)
item NUNZIATA, SCHYLER - Animal And Plant Health Inspection Service (APHIS)
item Jaiswal, Namrata
item Ling, Kai-Shu
item MAVRODIEVA, VESSELA - Animal And Plant Health Inspection Service (APHIS)
item RIVERA, YAZMIN - Animal And Plant Health Inspection Service (APHIS)
item NAKHLA, MARK - Animal And Plant Health Inspection Service (APHIS)

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2022
Publication Date: 11/1/2022
Citation: Abrahamian, P., Cai, W., Nunziata, S.O., Jaiswal, N., Ling, K., Mavrodieva, V.A., Rivera, Y., Nakhla, M.K. 2022. Effectiveness of disinfectants to manage the emerging tomato brown rugose fruit virus in greenhouse tomato. Phytopathology. https://doi.org/10.1094/PHYTO-112-11-S3.1.
DOI: https://doi.org/10.1094/PHYTO-112-11-S3.1

Interpretive Summary: n/a

Technical Abstract: Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus that threatens tomato and pepper crops around the world. Genetic resistance is an effective means for tobamoviruses control. However, ToBRFV causes infection and often with severe symptoms in tomato cultivars due to overcoming the popular Tm-2^2 resistance gene. Currently, genetic diversity studies for ToBRFV are mostly biased towards Dutch isolates in the public database. In this study, we sequenced and characterized additional isolates to study the diversity of this emerging virus among the worldwide genome collection. A total of 22 isolates, collected from the U.S., Mexico and Peru were characterized and compared to 123 full genomes publicly available. High-throughput sequencing was used to obtain near complete genomes. Virus read depth ranged between 78x to 773,154x and a minimum sequence coverage of 99.4%. Genetic diversity across the isolates was low overall, with the lowest and highest diversity observed in the coat protein and movement protein gene, respectively. Genetic diversity parameters showed that the ToBRFV is undergoing a population expansion, negative selection pressure, with random mutations arising across the genomes. Based on phylogenetic analysis, the majority (N=138) of the isolates belong to one of three genetically differentiated groups. The results of this study agree with previous genomic studies indicating low diversity and suggesting a similar potential origin in South America. Currently deployed molecular assays for diagnostics should remain effective given the low genetic diversity of ToBRFV.