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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Publications at this Location » Publication #393634
Research Project: Integrated Pest Management of Mosquitoes and Biting Flies

Location: Mosquito and Fly Research

Title: Resistance breaking insecticidal activity of new spatial insecticides against Aedes aegypti

Author
item RICHOUX, GARY - University Of Florida
item YANG, LIU - University Of Florida
item JIANG, SHIYAO - University Of Florida
item Norris, Edmund
item Linthicum, Kenneth - Ken
item BLOOMQUIST, JEFFREY - University Of Florida

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/12/2021
Publication Date: 2/25/2021
Citation: Richoux, G.M., Yang, L., Jiang, S., Norris, E.J., Linthicum, K., Bloomquist, J. 2021. Resistance breaking insecticidal activity of new spatial insecticides against Aedes aegypti. Emerging Pathogens Institute Research Day. p.74-75.

Interpretive Summary:

Technical Abstract: Because the literature around N-aryl amide derivatives as spatially-acting insecticides remains relatively unexplored, we synthesized eighty-nine analogues and screened them for mortality against the insecticide susceptible Orlando (OR) strain of Aedes aegypti using a vapor exposure glass tube assay. Of the screened compounds, twenty-two produced >92% mortality at twenty-four hours and warranted further investigation to determine LC50 values. Fifteen of these analogs had LC50 values within two orders of magnitude of transfluthrin, and of significant interest, N-(2,6-dichloro-4-(trifluoromethyl)phenyl)-2,2,3,3,3-pentafluoropropanamide was nearly as potent as transfluthrin and exhibited greater toxicity than metofluthrin when screened against OR Ae. aegypti. Select compounds were screened against the insecticide resistant, Puerto Rico (PR) strain of Ae. aegypti and it was discovered that not only did these N-arylamides typically show little resistance, some such as N-(2,6-dichloropyridin-4-yl)-2,2,3,3,4,4,4-heptafluorobutanamide and 2,2,3,3,4,4,4-heptafluoro-N-(3,4,5-trifluorophenyl) butanamide were actually more potent against the PR mosquitoes. Due to this promising insecticidal activity, five compounds were administered orally to mice to determine acute oral rodent toxicity. All five compounds were found to have mouse oral toxicity LD50 values well above the minimum level as set by the Innovative Vector Control Consortium (50 mg/kg).1 In addition to the promising biological activity documented here, we report the structure-activity relationship analysis used to guide the derivatization approach taken and to further inform future efforts in the development of N-arylamides as potential resistance-breaking, spatially-acting insecticides.