Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #393694
Research Project: Development of Novel Cottonseed Products and Processes

Location: Commodity Utilization Research

Title: Real-time quantitative PCR analysis of gene expression in human colon cancer cells treated with LPS

Author
item Cao, Heping

Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Database / Dataset
Publication Acceptance Date: 4/18/2022
Publication Date: 4/18/2022
Citation: Cao, H. 2022. Real-time quantitative PCR analysis of gene expression in human colon cancer cells treated with LPS. NIH Gene Expression Omnibus (GEO) Database. GSE200980.

Interpretive Summary: Colon cancer is one of the deadliest diseases in the world with approximately 4.0% of the people developing colorectal cancer in their lifetime. It is urgently needed to fully understand the mechanism of developing colon cancer and explore ways to ease the burden of the healthcare crisis. Lipopolysaccharide (LPS) is a bacterial endotoxin proposed to have antitumor effect. Previous studies have explored the effects of LPS on gene expression in colon cancer cells, but they were focused on very few targets. The objective of this study was to survey the effects of LPS on gene expression of 55 molecular targets in human colon cancer cells. This technical note describes the dataset of relative quantitation of 55 mRNAs in human colon cancer cell line COLO 225 generated by the SYBR Green qPCR method.

Technical Abstract: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. Colon bacteria contribute to LPS which promotes colon cancer metastasis by enhancing tumor cell adhesion, intravasation, and extravasation. Previous studies explored the effects of LPS on gene expression in colon cancer cells, but they were focused on very few targets. The objective of this study was to survey the effects of LPS on gene expression of 55 molecular targets in human colon cancer cells. This technical note described the dataset of relative quantitation of 55 mRNAs in human colon cancer cell line COLO 225 generated by the SYBR Green qPCR method.