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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #394547
Research Project: Genetic Mechanisms and Improvement of Insect Resistance in Wheat, Barley, and Sorghum

Location: Peanut and Small Grains Research Unit

Title: Characterization of Rsg3, a novel greenbug resistance gene from the Chinese barley landrace PI 565657

Author
item Xu, Xiangyang
item Mornhinweg, Dolores - Do
item Bai, Guihua
item Li, Genqiao
item BIAN, RUOLIN - Kansas State University
item Bernardo, Amy
item Armstrong, John

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/19/2022
Publication Date: 12/8/2022
Citation: Xu, X., Mornhinweg, D.W., Bai, G., Li, G., Bian, R., Bernardo, A.E., Armstrong, J.S. 2022. Characterization of Rsg3, a novel greenbug resistance gene from the Chinese barley landrace PI 565657. The Plant Genome. 16(1). Article e20287. https://doi.org/10.1002/tpg2.20287.
DOI: https://doi.org/10.1002/tpg2.20287

Interpretive Summary: Greenbug (Schizaphis graminum Rondani) is a pest posing a serious threat to cereal production worldwide, and yield losses caused by greenbug are predicted to increase because of global warming. Although insecticides are available to control greenbug, the considerable costs, safety concerns, and potential contamination of the environment make chemical control less attractive than host plant resistance. To date, only a few barley greenbug resistance genes have been reported and new genes are urgently needed because of the continuous occurrence of novel greenbug biotypes. PI 565676 is a landrace collected from Henan province of China and exhibits high resistance to several predominant greenbug biotypes. The greebug resistance gene in PI 565676, designated Rsg3, was located on the long arm of chromosome 3H. Rsg3 is a new greenbug resistance gene that may play an important role in developing resilient barley cultivars. Two user-friendly, high throughput KASP markers were developed for the rapid introgression of Rsg3 into locally adapted barley cultivars.

Technical Abstract: Greenbug (Schizaphis graminum Rondani) is a pest posing a serious threat to cereal production worldwide. Yield losses caused by greenbug are predicted to increase because of global warming. To date, only a few barley greenbug resistance genes have been reported and new genes are urgently needed because of the continuous occurrence of novel greenbug biotypes. PI 565676 is a landrace collected from Henan province of China, and exhibits high resistance to several predominant greenbug biotypes. A recombinant inbred line (RIL) population derived from the cross PI 565676 × Weskan was evaluated for response to greenbug biotypes E and F, and genotyped using the single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing. Gene mapping placed a greenbug resistance gene in PI 565676, designated Rsg3, to an interval of 93,140 bp between 667,558,306 to 667,651,446 bp on the long arm of chromosome 3H. Four high confidence genes were annotated in this region with one encoding a leucine-rich repeat-containing protein. Allelism test indicated that the greenbug resistance gene in PI 565676 is independent of the Rsg1 locus, with estimated recombination frequency of 12.85 ± 0.20% and genetic distance of 13.14 ± 0.21 cM between the two loci. Therefore, Rsg3 in PI 565676, represents a new locus for greenbug resistance. Two SNPs flanking Rsg3 were converted to KASP markers which can be used to tag Rsg3 in barley breeding.