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ARS Home » Southeast Area » Auburn, Alabama » Aquatic Animal Health Research » Research » Publications at this Location » Publication #394671
Research Project: Integrated Research to Improve Aquatic Animal Health in Warmwater Aquaculture

Location: Aquatic Animal Health Research

Title: Analysis of variation of immunoglobulin (Ig) M transcripts in channel catfish vaccinated with extracellular proteins of virulent Aeromonas hydrophila

Author
item Zhang, Dunhua
item Lange, Miles
item Shoemaker, Craig
item Beck, Benjamin

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Database / Dataset
Publication Acceptance Date: 10/11/2021
Publication Date: 3/2/2022
Citation: Zhang, D., Lange, M.D., Shoemaker, C.A., Beck, B.H. 2022. Analysis of variation of immunoglobulin (Ig) M transcripts in channel catfish vaccinated with extracellular proteins of virulent Aeromonas hydrophila. National Center for Biotechnology Information (NCBI).

Interpretive Summary:

Technical Abstract: Immunoglobulin (Ig) M is the major antibodies produced in Channel catfish (Ictalurus punctatus) against invading pathogens/antigens. Vaccination of the fish with extracellular proteins (ECP) of virulent Aeromonas hydrophila (vAh, a severe disease-causing bacterial pathogens) protected fish against the establishment of pathogenesis. IgM transcripts (cDNA) from vaccinated/disease resistant fish were cloned and sequenced. Sequence data revealed differential expression of IgM isotypes (variants) between vaccinated fish and unvaccinated control fish. Vaccinated fish produced specific IgM transcripts in response to vAh immunogens. Characterized sequences of these IgM isotypes were deposited in the National Center for Biotechnology Information (NCBI) database under GenBank accession numbers: MZ803140 - MZ803182, MZ773892, and MZ773893.