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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #394729
Research Project: Development of Novel Cottonseed Products and Processes

Location: Commodity Utilization Research

Title: Real-time quantitative PCR analysis of gene expression in mouse macrophages treated with bacterial endotoxin lipopolysaccharide

Author
item Cao, Heping

Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Database / Dataset
Publication Acceptance Date: 6/2/2022
Publication Date: 6/3/2022
Citation: Cao, H. 2022. Real-time quantitative PCR analysis of gene expression in mouse macrophages treated with bacterial endotoxin lipopolysaccharide. NIH Gene Expression Omnibus (GEO) Database. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE204820

Interpretive Summary: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. LPS is heat-stable endotoxin functioning in protection of gram-negative bacteria against the action of bile salts and lipophilic antibiotics. It plays a key role in septic shock in humans by inducing a strong immune response in normal mammalian cells. It is well documented that the bacterial endotoxin LPS rapidly stimulates TTP gene expression at both mRNA and protein levels but less is known about its effect on other TTP family gene expression. The objective was to evaluate LPS effects on gene expression involved in inflammatory responses, lipid biosynthesis, glucose transport and insulin signaling pathway in mouse macrophages. This technical note describes the dataset of LPS on relative levels of 27 mRNAs in mouse RAW264.7 macrophages generated by the SYBR Green qPCR method.

Technical Abstract: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. It is well documented that the bacterial endotoxin LPS rapidly stimulates TTP gene expression at both mRNA and protein levels but less is known about its effect on other TTP family gene expression. The objective was to evaluate LPS effects on gene expression involved in inflammatory responses, lipid biosynthesis, glucose transport and insulin signaling pathway in mouse macrophages. Mouse RAW264.7 macrophages were treated with multiple concentrations of LPS for 2-24 h. qPCR analyzed the expression of 27 genes coding for anti-inflammatory tristetraprolin family (TTP/ZFP36), proinflammatory cytokine, lipid biosynthesis (DGAT), glucose transporter (GLUT) and insulin signaling genes. This technical note describes the dataset of LPS on relative levels of 27 mRNAs in mouse RAW264.7 macrophages generated by the SYBR Green qPCR method.