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Research Project: Microbiota and Nutritional Health

Location: Children's Nutrition Research Center

Title: Non-targeted metabolomics of cooked cowpea (Vigna unguiculate) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary platforms

Author
item SAYRE-CHAVEZ, BROOKE - Colorado State University
item BAXTER, BRIDGET - Colorado State University
item BROECKLING, COREY - Colorado State University
item MUNOZ-AMATRIAIN, MARIA - Colorado State University
item MANARY, MARK - Children'S Nutrition Research Center (CNRC)
item RYAN, ELIZABETH - Colorado State University

Submitted to: Food Chemistry: Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/11/2022
Publication Date: 2/14/2022
Citation: Sayre-Chavez, B., Baxter, B., Broeckling, C.D., Munoz-Amatriain, M., Manary, M., Ryan, E.P. 2022. Non-targeted metabolomics of cooked cowpea (Vigna unguiculate) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary platforms. Food Chemistry: Molecular Sciences. 4. Article 100087. https://doi.org/10.1016/j.fochms.2022.100087.
DOI: https://doi.org/10.1016/j.fochms.2022.100087

Interpretive Summary: Legumes are global staple foods with many unique nutrients. This study examined three blackeyed-pea varieties and one pigeon pea from Ghana using two different analytical methods and workflows. This study identified novel cowpea and pigeon pea food metabolites that can be used to quantify legume intake in the diet.

Technical Abstract: Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene1,2 diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people.