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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #396334
Research Project: Genetic Improvement of Biotic and Abiotic Stress Tolerance and Nutritional Quality in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research

Title: Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse

Author
item LI, HANWEI - China Agricultural University
item ZHANG, FUPING - China Agricultural University
item ZHAO, JIXIN - Kansas State University
item Bai, Guihua
item St Amand, Paul
item Bernardo, Amy
item NI, ZHONGFU - China Agricultural University
item XUN, QIXIN - China Agricultural University
item SU, ZHENQI - Kansas State University

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/10/2022
Publication Date: 3/31/2022
Citation: Li, H., Zhang, F., Zhao, J., Bai, G., St Amand, P.C., Bernardo, A.E., Ni, Z., Xun, Q., Su, Z. 2022. Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse. Journal of Theoretical and Applied Genetics. 135.1867–1877. https://doi.org/10.1007/s00122-022-04080-5.
DOI: https://doi.org/10.1007/s00122-022-04080-5

Interpretive Summary: Fusarium head blight (FHB) causes significant reductions in grain yield and quality worldwide. To identify new sources of FHB resistance, a Chinese wheat cultivar, Ji5265, was identified with high FHB resistance and used to develop a recombinant inbred population to map resistance genes in the cultivar. We mapped a major effect gene, QFhb-2DL, on the long arm of chromosome 2D in Ji5265. Two KASP markers were developed and validated to be diagnostic for QFhb-2DL in a collection of 2,065 wheat accessions. QFhb-2DL is a novel major QTL for FHB resistance and the two KASP markers can be used for marker-assisted selection of QFhb-2DL.

Technical Abstract: Fusarium head blight (FHB) is a serious disease in wheat (Triticum aestivum) and causes significant reductions in grain yield and quality worldwide. Breeding for FHB resistance is the most effective strategy to minimize the losses caused by FHB; therefore, identification of major quantitative trait loci (QTLs) conferring FHB resistance and development of diagnostic markers for the QTLs are prerequisites for marker-assisted selection (MAS). Ji5265 is a Chinese wheat cultivar resistant to FHB in multiple environments. An F6 population of 179 recombinant inbred lines (RILs) was developed from Ji5265 × Wheaton. The population was genotyped by genotyping-by-sequencing (GBS) and phenotyped for FHB Type II resistance in greenhouses. A major QTL, designated QFhb-2DL, was mapped in a 6.8 Mb region between the markers GBS10238 and GBS12056 on the long arm of chromosome 2D in Ji5265 and explained ~30% of the phenotypic variation for FHB resistance. The effect of QFhb-2DL on FHB resistance was validated using near-isogenic lines (NILs) derived from residual heterozygotes from an F6 RIL of Ji5265 × Wheaton. The two flanking markers were converted into Kompetitive allele specific PCR (KASP) markers (KASP10238 and KASP12056) and validated to be diagnostic in a collection of 2,065 wheat accessions. These results indicate that QFhb-2DL is a novel major QTL for resistance to FHB spread within a spike (Type II) and the two KASP markers can be used for MAS to improve wheat FHB resistance in wheat breeding programs.