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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #396402
Research Project: Surveillance, Pathogen Biology, and Host Resistance of Cereal Rusts

Location: Cereal Disease Lab

Title: The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Author
item YU, GUOTAI - King Abdullah University Of Science And Technology
item MATNY, OADI - University Of Minnesota
item GOURDOUPIS, SPYRIDON - King Abdullah University Of Science And Technology
item ALJEDAANI, FATIMAH - King Abdullah University Of Science And Technology
item WANG, YAN - University Of Tubingen
item NÜRNBERGER, THORSTEN - University Of Tubingen
item JOHNSON, RYAN - University Of Minnesota
item CREAN, EMMA - University Of Cologne
item SAUR, ISABEL - University Of Cologne
item GARDENER, CATHERINE - King Abdullah University Of Science And Technology
item YUE, YAJUAN - John Innes Center
item KANGARA, NGONIDZASHE - John Innes Center
item STEUERNAGEL, BURKHARD - John Innes Center
item HAYTA, SADIYE - John Innes Center
item SMEDLEY, MARK - John Innes Center
item HARWOOD, WENDY - John Innes Center
item PATPOUR, MEHRAN - Aarhus University
item WU, SHUANGYE - Kansas State University
item POLAND, JESSE - King Abdullah University Of Science And Technology
item JONES, JONATHAN - Sainsbury Laboratory
item REUBER, T - Sainsbury Laboratory
item RONEN, MOSHE - Tel Aviv University
item SHARON, AMIR - Tel Aviv University
item Rouse, Matthew
item Xu, Steven
item HOLUŠOVÁ, KATERINA - Czech Academy Of Sciences
item BARTOŠ, JAN - Czech Academy Of Sciences
item MOLNÁR, ISTVÁN - Czech Academy Of Sciences
item KARAFIÁTOVÁ, MIROSLAVA - Czech Academy Of Sciences
item HIRT, HERIBERT - King Abdullah University Of Science And Technology
item BLILOU, IKRAM - King Abdullah University Of Science And Technology
item JAREMKO, LUKASZ - King Abdullah University Of Science And Technology
item DOLEŽEL, JAROSLAV - Czech Academy Of Sciences
item STEFFENSON, BRIAN - University Of Minnesota
item WULFF, BRANDE - John Innes Center

Submitted to: Nature Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2023
Publication Date: 5/22/2023
Citation: Yu, G., Matny, O., Gourdoupis, S., Aljedaani, F.R., Wang, Y.L., Nürnberger, T., Johnson, R., Crean, E.E., Saur, I.M., Gardener, C., Yue, Y., Kangara, N., Steuernagel, B., Hayta, S., Smedley, M., Harwood, W., Patpour, M., Wu, S., Poland, J., Jones, J.D., Reuber, T.L., Ronen, M., Sharon, A., Rouse, M.N., Xu, S.S., Holušová, K., Bartoš, J., Molnár, I., Karafiátová, M., Hirt, H., Blilou, I., Jaremko, L., Doležel, J., Steffenson, B.J., Wulff, B.B. 2023. The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase. Nature Genetics. 55:921-926. https://doi.org/10.21203/rs.3.rs-1820134/v1.
DOI: https://doi.org/10.21203/rs.3.rs-1820134/v1

Interpretive Summary: Wheat is grown on over 37 million acres in the United States. The rust diseases of wheat can cause devastating yield losses. In order to mitigate wheat stem rust, disease-resistant wheat varieties need to be developed. We cloned the stem rust resistance gene Sr43, which was crossed into bread wheat from the wild grass Thinopyrum ponticum. Sr43 encodes a protein kinase fused to two domains of unknown function. Transgenic expression of Sr43 in wheat conferred high levels of resistance to a wide range of isolates of the pathogen causing stem rust, highlighting the potential value of Sr43 in resistance breeding and engineering. The identification of the DNA sequence that confers Sr43 resistance can be used in wheat breeding to develop rust-resistant wheat varieties for the United States.

Technical Abstract: To safeguard bread wheat against pests and diseases, breeders have introduced over 200 resistance genes into its genome, thus nearly doubling the number of designated resistance genes in the wheat gene pool. Isolating these genes facilitates their fast-tracking in breeding programs and incorporation into polygene stacks for more durable resistance. We cloned the stem rust resistance gene Sr43, which was crossed into bread wheat from the wild grass Thinopyrum ponticum. Sr43 encodes a protein kinase fused to two domains of unknown function. The gene, which is unique to the Triticeae, appears to have arisen through a gene fusion event 6.7 to 11.6 million years ago. Transgenic expression of Sr43 in wheat conferred high levels of resistance to a wide range of isolates of the pathogen causing stem rust, highlighting the potential value of Sr43 in resistance breeding and engineering.