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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #396952
Research Project: Optimizing Nutrient Management and Efficiency of Beef Cattle and Swine

Location: Nutrition, Growth and Physiology

Title: Supplementation of one-carbon metabolites to beef heifers during early gestation effects on fetal liver and muscle mitochondrial oxygen consumption rate ratios at day 63 of gestation

Author
item KING, L - North Dakota State University
item SYRING, J - North Dakota State University
item ENTZIE, Y - North Dakota State University
item HIRCHERT, M - North Dakota State University
item Crouse, Matthew
item CATON, J - North Dakota State University
item DAHLEN, C - North Dakota State University
item WARD, A - North Dakota State University

Submitted to: State University Ag Report
Publication Type: Other
Publication Acceptance Date: 9/30/2022
Publication Date: 10/8/2022
Citation: King, L.E., Syring, J., Entzie, Y., Hirchert, M., Crouse, M.S., Caton, J.S., Dahlen, C.R., Ward, A.K. 2022. Supplementation of one-carbon metabolites to beef heifers during early gestation effects on fetal liver and muscle mitochondrial oxygen consumption rate ratios at day 63 of gestation. 2022 North Dakota Livestock Research Report. 77-79. Available: https://www.ndsu.edu/agriculture/extension/publications/2022-north-dakota-livestock-research-report

Interpretive Summary:

Technical Abstract: The objective of this experiment was to investigate the effects of one-carbon metabolite supplementation during early gestation on beef cattle fetal liver and muscle cellular respiration. The experiment utilized 72 angus-crossbred heifers that were housed at the Animal Nutrition Physiology Center at North Dakota State University from June 2021 until October 2021. The experimental design has treatments arranged as a 2 × 2 factorial with two levels of feed intake (planes of nutrition) and two levels of strategic OCM supplementation. The heifers were assigned to one of four nutritional treatments: control intake with-out OCM supplementation (CON - OCM), CON with OCM supplementation (CON + OCM), restricted intake without OCM supplementation (RES - OCM), or RES with OCM supplementation (RES + OCM). At d 63 of gestation fetal liver and muscle tissues were collected and oxygen consumption rate (OCR) was measured. Respiratory control ratio (RCR) was determined using state 3 and 4 oxygen consumption rate values from mitochondrial respiration assays. No treatment, gain, or interaction effects were observed for RCR in liver and muscle tissue. Similarly, when RCR values were normalized to a control, no treatment, gain or interaction effects were seen. Results show that more assays in later phases of the project are necessary to determine effects of OCM supplementation on mitochondrial oxygen consumption rates. All P-values were above 0.05.