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Submitted to: Advances in Enzyme Research
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 3/28/2023 Publication Date: 3/31/2023 Citation: Wong, D., Batt Throne, S.B., Orts, W.J. 2023. Combinatorial enzyme approach to convert wheat insoluble arabinoxylan to bioactive oligosaccharides. Advances in Enzyme Research. 11(1):1-10. https://doi.org/10.4236/aer.2023.111001. DOI: https://doi.org/10.4236/aer.2023.111001 Interpretive Summary: The major component in biomass plant cell wall is a complex structure comprised of heterogeneous mixtures of branched and highly hydrated polysaccharides. The polymeric chain can chemically or enzymatically break down into small oligosaccharide fragments consisting of variable sizes and side groups. The structural diversity of oligosaccharide fragments would express into different reactivity and functional properties. Using the technology developed by this lab, specific enzymes were applied to produce libraries of oligosaccharides from wheat insoluble arabinoxylan after high pressure hot water pretreatment. Repeated fractionation and screening resulted in the isolation of active oligo species with antimicrobial activity on a test organism in liquid culture. The active oligo species may be useful as alternatives or a new source of functional preservatives. Technical Abstract: Combinatorial enzyme technology was applied for the bioconversion of wheat insoluble arabinoxylan to oligosaccharides of structural variants. The digestive products were fractionated by Bio-Gel P4 column and screened for bioactivity. One fraction pool was observed to exhibit antimicrobial property resulting in the suppression of cell growth of the test organism ATCC 8739 E. coli. It has a MIC value of 1.5% (w/v, 35oC, 20 hr) and could be useful as antimicrobial growth promoter or as a new source of high-value preservative. The present results further confirm the science and useful application of combinatorial enzyme approach. |
