Location: Plant Stress and Germplasm Development Research
Title: Pedigreed sorghum mutant library as a public resource for discovering trait for breeding and targets for genome editingAuthor
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 9/7/2022 Publication Date: 9/9/2022 Citation: Xin, Z., Chen, J. 2022. Pedigreed sorghum mutant library as a public resource for discovering trait for breeding and targets for genome editing. John A. Browse Symposium on Plant Lipids and Beyond. Interpretive Summary: Technical Abstract: Mutation breeding is a classical and effective approach to generate diversity and create novel traits. A manageable number of heavily mutated lines can yield large numbers of mutations covering the entire genome and produce a plethora of phenotypes. We have established a Pedigreed Mutant Library in the sorghum inbred line BTx623 by mutagenizing the seeds with ethyl methane sulfonate (EMS). This library has 6,400 M4 seed pools and possesses a great diversity of mutant phenotypes. We sequenced 1,256 lines randomly selected from the mutant library and generated >8.8 million EMS-induced mutations. Over 16,000 genes have at least one knockout mutation. There are only 517 small genes that have no mutation. Furthermore, we have established an effective bioinformatic pipeline to identify the causal mutations through bulk-segregant-analysis (BSA) of the whole genome sequencing data of the pooled mutant plants selected from F2 populations. Once an F2 backcrossed population is established, the cost to identify the causal mutation is under $200 in NGS sequencing. The sorghum mutant library will be a useful resource for creating new traits for breeding and informing targets for genome editing. |
