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Title: TRI10: A NEW GENE IN THE TRICHOTHECENE GENE CLUSTER IN FUSARIUM SPOROTRICHIOIDES

Author
item BEREMAND MARIAN - TEXAS A & M UNIV
item Hohn, Thomas

Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/26/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Fusarium sporotrichioides produces a spectrum of trichothecenes with the primary metabolite being T-2 toxin. Genetically, the synthesis of these sesquiterpenoid mycotoxins is determined, in part, by pathway genes that are organized in a large gene cluster. Preliminary DNA sequence information indicated the presence of two small open reading frames, ORF-1 and ORF-2, downstream from the Tri5 gene which encodes the pathway enzyme, trichodiene synthase. Northern analyses conducted with DNA probes within and/or overlapping these two ORFs demonstrated that each probe hybridized to a transcript approximately 1400 bases in length. A pair of primers within ORF-2 has also yielded a PCR product of identical size from both genomic DNA and DNA prepared from a cDNA library. In addition, the pattern of expression of this transcript parallels that of the other known pathway genes located in the trichothecene gene cluster. These results indicate that we have identified a new gene, Tri10, within the trichothecene gene cluster. Experiments are underway to determine the complete sequence of the Tri10 cDNA and to investigate the function of this gene via gene disruption.