Skip to main content
ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #397287
Research Project: Advancing Knowledge of the Biology and Etiology of Bacterial Plant Pathogens Towards Management Strategies

Location: Emerging Pests and Pathogens Research

Title: Complete genome sequence resource for xanthomonas hortorum isolated from Greek oregano

Author
item GONZALEZ-TOBON, JULIANA - Cornell University
item HELMANN, TYLER - Former ARS Employee
item DAUGHTRY, MARGERY - Cornell University
item Stodghill, Paul
item Filiatrault, Melanie

Submitted to: Phytopathology
Publication Type: Rapid Release Publication
Publication Acceptance Date: 3/9/2023
Publication Date: 10/23/2024
Citation: Gonzalez-Tobon, J., Helmann, T., Daughtry, M., Stodghill, P., Filiatrault, M.J. 2024. Complete genome sequence resource for xanthomonas hortorum isolated from Greek oregano. Phytopathology. 107:3259-3263. https://doi.org/10.1094/PDIS-10-22-2399-A.
DOI: https://doi.org/10.1094/PDIS-10-22-2399-A

Interpretive Summary: Xanthomonas hortorum is a bacterial phytopathogen that affects over 65 plant species including agricultural crops, horticultural plants, and wild plants. This devastating plant pathogen can lead to total loss of a crop when environmental conditions are favorable. Unfortunately, some knowledge gaps remain for this pathogen, such as the extent of host range, detection, and control methods. There is a significant need to conduct comprehensive comparative genomics analyses of this species, especially because there have been some recent taxonomical changes but there are only a few quality genomes available for this pathogen. Here we isolated a bacterium causing disease on Greek oregano. Using whole genome sequencing we identified the pathogen as Xanthomonas hortorum. The results provide the identification of a novel isolate from new natural hosts and new genomic data for X. hortorum which can be used as a resource that will help in the identification of bacterial factors for diagnostics, factors involved in host-interactions, or factors essential for pathogenicity.

Technical Abstract: In spring 2019, necrotic leaf spots were detected on Greek oregano (Origanum vulgare var. hirtum) plants in a commercial greenhouse operation. An isolate was recovered from the diseased plants. Partial 16S rRNA sequencing and multi locus sequence analysis (MLSA) revealed the isolate was a Xanthomonas but proved insufficient to identify the species with certainty. Therefore, whole genome sequencing using both Nanopore and Illumina technologies was performed. Here, we report the complete and annotated genome sequence of Xanthomonas hortorum strain108, which was originally isolated from Greek Oregano in Long Island, NY, U.S.A.