Skip to main content
ARS Home » Research » Publications at this Location » Publication #397464
Research Project: Intervention Strategies to Respond, Control, and Eradicate Foot-and-Mouth Disease Virus (FMDV)

Location: Location not imported yet.

Title: Foot-and-mouth disease virus SAT1 sequences purified from co-infected Cape buffalo in Kenya

Author
item PALINSKI, RACHELC - Kansas State University
item SANGULA, ABRAHAM - Kenya Fmd Laboratory- Wrlfmd
item GAKUYA, FRANCIS - Wildlife Research And Training Institute
item Bertram, Miranda
item PAUSZEK, STEVEN - Animal And Plant Health Inspection Service (APHIS)
item Hartwig, Ethan
item Smoliga, George
item OBANDA, VINCENT - Wildlife Research And Training Institute
item VANDERWAAL, KIMBERLY - University Of Minnesota
item Arzt, Jonathan
item OMONDI, GEORGE - University Of Minnesota

Submitted to: Microbiology Resource Announcements
Publication Type: Other
Publication Acceptance Date: 8/17/2022
Publication Date: 9/12/2022
Citation: Palinski, R., Sangula, A., Gakuya, F., Bertram, M.R., Pauszek, S., Hartwig, E.J., Smoliga, G.R., Obanda, V., Vanderwaal, K., Arzt, J., Omondi, G. 2022. Foot-and-mouth disease virus SAT1 sequences purified from co-infected Cape buffalo in Kenya. Microbiology Resource Announcements. https://doi.org/10.1128/mra.00584-22.
DOI: https://doi.org/10.1128/mra.00584-22

Interpretive Summary: Foot-and-mouth disease virus (FMDV) is an important viral disease affecting animal health and economics through impacts on agriculture and trade. African Cape Buffalo are a reservoir for FMD, and can be infected with multiple serotypes without having clinical signs of disease. This paper reports serotype SAT1 sequences from subclinically infected buffalo in Kenya. Additionally, we report SAT1 sequences acquired through plaque purification of samples from buffalo that were infected with both SAT1 and SAT2. Plaque purification separates the different viruses in a sample, resulting in more accurate sequences than can be obtained by directly sequencing a sample containing multiple viruses.

Technical Abstract: Forty-nine novel foot-and-mouth disease virus SAT1 genomes were acquired from oropharyngeal fluid samples from asymptomatic African Cape buffalo in Kenya in 2016. Sequences were from primary passage or plaque purified SAT1/SAT2 dual infected samples These genomes are important to elucidation of the molecular epidemiology of FMDV persistent and subclinical infections.