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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #397955
Research Project: Discovery and Improvement of Traits to Enhance Sorghum as a Multiple Purpose Crop

Location: Plant Stress and Germplasm Development Research

Title: Registration of 252 sequenced sorghum mutants as a community reverse genetic resource

Author
item Xin, Zhanguo
item JIAO, YINPING - Texas Tech University
item Burow, Gloria
item Hayes, Chad
item Chen, Junping
item Burke, John
item Pugh, Nicholas - Ace
item Ware, Doreen

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/20/2023
Publication Date: 5/26/2023
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Burke, J.J., Pugh, N.A., Ware, D. 2023. Registration of 252 sequenced sorghum mutants as a community reverse genetic resource. Journal of Plant Registrations. 17(3):599-604. https://doi.org/10.1002/plr2.20296.
DOI: https://doi.org/10.1002/plr2.20296

Interpretive Summary: Sequenced mutant library is a very important resource for studying gene function and select new traits for crop improvement. Scientists from ARS and Texas Tech University have developed a pedigreed mutant library through chemical mutagenesis from the sorghum inbred line BTx623 that is used to generate the first reference genome sequence in sorghum. The mutant library displayed a wide diversity of phenotypes, including potential traits of significant agronomic value. A selection of 252 lines were sequenced to an average depth of 16x with pair-ends sequencing on Illumina Highseq. This endeavor resulted in the detection and cataloguing of more than 1.8 million canonical EMS-induced mutations (or variants). The variants were distributed across the sorghum genome uniformly, with 24,757 genes were affected by consequential mutations that may alter the gene function. Mutations in genes of interest can be searched online through SorghumBase (https://www.sorghumbase.org). These sequenced mutants can be ordered through GRIN (https://www.ars-grin.gov/ ). This research is important to sorghum breeders and scientists interested in genetics, genomics, and physiology.

Technical Abstract: Induced mutagenesis is a powerful approach to generate variations that can be used towards the elucidation of gene function and create new traits for crop improvement. We have developed a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds from the sorghum inbred line BTx623. The mutant library displayed a wide diversity of phenotypes, including potential traits of significant agronomic value. A selection of 252 lines were sequenced to an average depth of 16x with pair-ends sequencing on Illumina Highseq. This endeavor resulted in the detection and cataloguing of more than 1.8 million canonical EMS-induced mutations (or variants). The variants were distributed across the sorghum genome uniformly, with 24,757 genes were affected by consequential mutations that may alter the gene function. Mutations in genes of interest can be searched online through SorghumBase (https://www.sorghumbase.org). These sequenced mutants (Registration number from PIxxxx to xxxx) can be ordered through GRIN (https://www.ars-grin.gov/) with no associated fee.