Skip to main content
ARS Home » Pacific West Area » Kimberly, Idaho » Northwest Irrigation and Soils Research » Research » Publications at this Location » Publication #397963
Research Project: Development of Elite Sugar Beet Germplasm Enhanced for Disease Resistance and Novel Disease Management Options for Improved Yield

Location: Northwest Irrigation and Soils Research

Title: Combined omics approaches reveal distinct mechanisms of resistance and/or susceptibility in sugar beet double haploid genotypes at early stages of beet curly top virus infection

Author
item Galewski, Paul
item Majumdar, Raj
item Lebar, Matthew
item Strausbaugh, Carl
item Eujayl, Imad

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/7/2023
Publication Date: 10/9/2023
Citation: Galewski, P.J., Majumdar, R., Lebar, M.D., Strausbaugh, C.A., Eujayl, I.A. 2023. Combined omics approaches reveal distinct mechanisms of resistance and/or susceptibility in sugar beet double haploid genotypes at early stages of beet curly top virus infection. International Journal of Molecular Sciences. 24(19). Article 15013. https://doi.org/10.3390/ijms241915013.
DOI: https://doi.org/10.3390/ijms241915013

Interpretive Summary: Sugar beet is highly susceptible to Beet curly top virus (BCTV) which significantly reduces yield and sugar content. The severity of disease symptoms caused by BCTV primarily depends upon virus strain type and genetic background of sugar beets. Underlying mechanisms are unknown which cause some BCTV strains to produce strong disease symptoms while others do not. Using BCTV resistant and susceptible sugar beet genotypes produced at Kimberly, ID (USDA-ARS), natural infection with beet leafhoppers carrying different virus strains, and combination of molecular and biochemical tools, we demonstrate strain specific elements that could act as potential pathogenicity factors. The data presented here will help design precise mitigation strategies against BCTV and improve sugar yield in the future.

Technical Abstract: Sugar beet is an economically important crop in the United States (U.S.) and Europe and is susceptible to the Beet curly top virus (BCTV). In the western U.S., the predominant strains of BCTV in sugar beet include CA/Logan, Colorado, Severe, and Worland. Disease symptom severities depend upon factors such as virus strain/s, plant genotype, age at infection etc. The regulatory role of BCTV strain specific small non-coding RNAs (sncRNAs) and their interaction with the host is unclear. Using BCTV susceptible and resistant genotypes, natural infection, and global RNAseq, we demonstrate differential regulation of sugar beet genes by BCTV strain specific sncRNAs. Among detected sncRNAs, sncRNA_26 as an example was common to all four strains and showed higher negative correlation with EL10Ac7g16816 (UPF0554 protein) gene expression in the susceptible line. Whereas CA/Logan specific sncRNAs namely sncRNA_4, 20, 21 showed higher negative correlation with EL10Ac1g01206 (LRR protein), EL10Ac5g12605 (7-deoxyloganetic acid glucosyltransferase), and EL10Ac6g14074 (transmembrane emp24) expression in the susceptible line respectively. Peptidomics analysis identified putative small open reading frame (sORF) derived peptides from BCTV strains. The data presented here suggest that genome divergence among BCTV strains differentially affects the production of sncRNAs and small peptides which could potentially affect pathogenicity and disease symptom development.