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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #400025
Research Project: Genetic Improvement and Cropping Systems of Alfalfa for Livestock Utilization, Environmental Protection and Soil Health

Location: Plant Science Research

Title: Alfalfa gene editing

Author
item Curtin, Shaun
item POUDEL, KABITA - University Of Minnesota
item SCHEAFFER, CRAIG - University Of Minnesota
item Xu, Zhanyou
item Samac, Deborah - Debby

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/13/2023
Publication Date: 1/18/2023
Citation: Curtin, S.J., Poudel, K., Scheaffer, C.C., Xu, Z., Samac, D.A. 2023. Alfalfa gene editing. Plant and Animal Genome Conference. January 13-18, 2023. San Diego, California.

Interpretive Summary:

Technical Abstract: Advances in gene editing and Agrobacterium mediated transformation (AMT) have been important breakthroughs in crop functional genomics. Researchers previously confined to working with mutant collections of model plants can now readily generate mutant plants for any gene of interest. For alfalfa, except for a handful of transformable lab accessions, there is still a major bottleneck to wide-spread gene editing. Factors including genotype, plant health, Agrobacterium strain, tissue culture media, reagent composition, selectable marker, and selection agent require optimization for successful transformation. This presentation will address recent applications of gene editing in alfalfa and discuss current efforts to expand the range of editable alfalfa genotypes.