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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #400278
Research Project: Evaluation and Development of Improved Soybean Germplasm, Curation of USDA Accessions and Regional Evaluations of New Genotypes

Location: Crop Genetics Research

Title: Analysis of the mitochondrial genome sequences of Diaporthe longicolla (syn. Phomopsis longicolla) isolates causing Phomopsis seed decay in soybean

Author
item Li, Shuxian
item HU, XIAOJUN - Animal And Plant Health Inspection Service (APHIS)
item Song, Qijian
item Arias De Ares, Renee

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/9/2022
Publication Date: 1/11/2023
Citation: Li, S., Hu, X., Song, Q., Arias, R.S. 2023. Analysis of the mitochondrial genome sequences of Diaporthe longicolla (syn. Phomopsis longicolla) isolates causing Phomopsis seed decay in soybean. Plant and Animal Genome Conference. https://plan.core-apps.com/pag_2023/abstract/963d7d6c9252da3647857a6b5d0808ac.

Interpretive Summary:

Technical Abstract: Diaporthe longicolla (syn. Phomopsis longicolla) is an important seed-borne fungal pathogen and the primary cause of Phomopsis seed decay (PSD) in soybean [Glycine max (L.) Merrill]. PSD is one of the most devastating seed diseases reducing soybean seed quality worldwide. As part of a genome sequencing project on the fungal Diaporthe-Phomopsis complex, draft genomes of eight D. longicolla isolates were sequenced and assembled using Spades program. Sequences of mitochondrial genomes were extracted and analyzed. The circular mitochondrial genomes range from 52,534 bp to 58, 280 bp long with GC content of 34%. Three isolates (PL7, PL 10, and PL 185E) had more introns than other isolates. A total of 14 common protein genes, 23 tRNA and two rRNA genes and ten introns (four in cox1, one in each cox2, nad5, atp6, rnl, nad1 and cob) were identified. In addition, sixteen transposons were also identified. Approximately, 6.4% of the mitochondrial genomes consist of repetitive elements. BLAST analysis against available mitochondrial sequence data at the NCBI showed that the mitochondrial genome sequences of eight D. longicolla isolates from this study were 99.83 to 100.00 % identical to previously reported D. longicolla type strain ATCC 60325 (accession KP137411.1). The mitochondrial genome sequences of D. longicolla will be useful for further study of the molecular basis of seed-borne pathogens causing seed disease in soybean, provide a valuable resource for development of molecular markers for detection, and for improvement of control strategies for Phomopsis seed decay.