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ARS Home » Midwest Area » Columbia, Missouri » Biological Control of Insects Research » Research » Publications at this Location » Publication #400393
Research Project: Biologically-Based Products for Insect Pest Control and Emerging Needs in Agriculture

Location: Biological Control of Insects Research

Title: Differential proteomics analysis unraveled mechanisms of Arma chinensis responding to improved artificial diet

Author
item ZOU, DEYU - Tianjin Academy Of Agricultural Sciences
item Coudron, Thomas
item WU, HUIHUI - Tianjin Agricultural University
item ZHANG, LISHENG - Chinese Academy Of Agricultural Sciences
item WANG, MENGQING - Chinese Academy Of Agricultural Sciences
item XU, WEIHONG - Tianjin Academy Of Agricultural Sciences
item XU, JINGYANG - Tianjin Academy Of Agricultural Sciences
item SONG, LIUXIAO - Tianjin Agricultural University
item XIAO, XEUZHUANG - Tianjin Agricultural University

Submitted to: Insects
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/28/2022
Publication Date: 7/2/2022
Citation: Zou, D., Coudron, T.A., Wu, H., Zhang, L., Wang, M., Xu, W., Xu, J., Song, L., Xiao, X. 2022. Differential proteomics analysis unraveled mechanisms of Arma chinensis responding to improved artificial diet. Insects. 13(7). Article 605. https://doi.org/10.3390/insects13070605.
DOI: https://doi.org/10.3390/insects13070605

Interpretive Summary: Arma chinensis Fallou is a predaceous stink bug that can effectively control many kinds of agricultural and forest pests, such as fall armyworm, cotton bollworm and Colorado potato beetle. An insect-free artificial diet comprising chicken egg, tuna fish and raw pig liver was developed for A. chinensis. Several biological characteristics were diminished for A. chinensis reared on the artificial diet compared to the pupae of Chinese oak silk moth. Changes in the formulation of the diet were made in response to the transcriptome results and tested using biological characteristics. Several parameters were improved over 6 generations, although the improved artificial diet remained inferior to the pupae of Chinese oak silk moth regarding egg viability, fecundity, body weight, and nymphal development time. The current study reported the differential proteomic analysis revealing the mechanism of A. chinensis responding to the improved artificial diet. This information will be used to optimize the formulation of the artificial diet and decrease the cost of mass rearing in A. chinensis.

Technical Abstract: The development of artificial diets could considerably simplify and reduce the cost of mass rearing of natural enemies compared to conventional rearing methods. However, improvement of artificial diets can be tedious, convoluted and often uncertain. For accelerating diet development, a better method that can offer informative feedback to target deficiencies in diet improvement is required. Our previous research demonstrated several biological characteristics were diminished in the insect predator, Arma chinensis Fallou, fed on an artificial diet formulated with the aid of transcriptomic methods compared to the Chinese oak silk moth pupae. The present study reports differential proteomic analysis by iTRAQ-PRM, which unravels the molecular mechanism of A. chinensis responding to improvements in the artificial diet. Our study provides multivariate proteomic data and provides comprehensive sequence information in studying A. chinensis. Further, the physiological roles of the differentially expressed proteins and pathways enable us to explain several biological differences between natural prey-fed and improved diet-fed A. chinensis, and subsequent proposed reformulation optimizations to artificial diets.