Toward cloning of H35 for Hessian fly resistance in hard winter wheat ‘Overland’

Authors: XU, XIAOTING - Kansas State University; XU, YUNFENG - Kansas State University; Chen, Ming-Shun; FRITZ, ALLAN - Kansas State University; Bai, Guihua

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary: Not required for Abstract.

Technical Abstract: Hessian fly (HF, Mayetiola destructor) is a serious pest of wheat (Triticum aestivum) in the U.S. and many other countries. Growing HF resistant cultivars is an effective approach to control the insect. To date, 37 HF resistance genes have been reported, but most of them were identified from wheat relatives and only 11 genes are from wheat. H35 (QHf.hwwg-3B) is one of these 11 wheat genes and was previously mapped on chromosome 3B using a recombinant inbred line population derived from ‘Overland’ x ‘Overley’. To finely map and eventually clone H35, three F6 recombinant inbred lines with residual heterozygotes at the H35 region were identified using two flanking markers KASP-3B210173 and KASP-3B4799538 for H35, and their 1,996 selfing progeny were screened to identify new recombinants within the H35 interval. Using the SNPs between Overland and Overley from exome capture data, 12 Kompetitive allele specific polymerase chain reaction (KASP) markers in the QTL region were designed and screened in the newly identified recombinants. Fine mapping using these new markers delimited the H35 interval to ~170 kb in which only four genes were annotated based on Chinese Spring Reference genome v2.1. One of the four candidate genes was differentially expressed between two near isogenic lines that carry the contrasting H35 alleles and is more likely the casual gene of H35. Future work will focus on the function validation of the candidate gene using gene editing.