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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #401483
Research Project: Enhancing Control of Stripe Rusts of Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: The interaction of two Puccinia striiformis f. sp. tritici effectors modulates the high-temperature seedling-plant resistance in wheat

Author
item BAO, XIYUE - Northwest A&f University
item HU, YANGSHAN - Northwest A&f University
item LI, YUXIANG - Northwest A&f University
item Chen, Xianming
item SHANG, HONGSHENG - Northwest A&f University
item HU, XIAOPING - Northwest A&f University

Submitted to: Molecular Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/31/2023
Publication Date: 10/3/2023
Citation: Bao, X., Hu, Y., Li, Y., Chen, X., Shang, H., Hu, X. 2023. The interaction of two Puccinia striiformis f. sp. tritici effectors modulates the high-temperature seedling-plant resistance in wheat. Molecular Plant Pathology. 24:1522-1534. https://doi.org/10.1111/mpp.13390.
DOI: https://doi.org/10.1111/mpp.13390

Interpretive Summary: Wheat cultivar Xiaoyan 6 confers high-temperature seeding-plant (HTSP) resistance to stripe rust. However, the molecular mechanism of pathogen effectors involved in the HTSP resistance remains unclear. In this study, we determined the interaction between two stripe rust pathogen effectors, PstCEP1 and PSTG_11208, through yeast-two-hybridization, bimolecular fluorescence complementation, and pull-down assays. Transient overexpression of PSTG_11208 enhanced the resistance under different temperature treatments. The interaction between PstCEP1 and PSTG_11208 inhibited the resistance enhancement by PSTG_11208. Furthermore, wheat apoplastic thaumatin-like protein 1 (TaTLP1) appeared recognizing pathogen invasion by interacting with PSTG_11208 to initiate the downstream defense response by the wheat pathogenesis-related protein TaPR1. Silencing TaTLP1 and TaPR1 separately or simultaneously reduced the HTSP resistance to the pathogen in the wheat cultivar. Furthermore, we found that PstCEP1 targeted wheat ferredoxin 1 (TaFd1), a homologous protein of rice OsFd1. Silencing TaFd1 affected the stability of photosynthesis in wheat plants, resulting in chlorosis on the leaves and reducing the HTSP resistance. Our findings revealed the synergistic mechanism of effector proteins in the process of pathogen infection.

Technical Abstract: Wheat cultivar Xiaoyan 6 (XY6) confers high-temperature seeding-plant (HTSP) resistance to Puccinia striiformis f. sp. tritici (Pst). However, the molecular mechanism of Pst effectors involved in the HTSP resistance remains unclear. In this study, we determined the interaction between two Pst effectors, PstCEP1 and PSTG_11208, through yeast-two-hybridization (Y2H), bimolecular fluorescence complementation (BiFC), and pull-down assays. Transient overexpression of PSTG_11208 enhanced the resistance under different temperature treatments. The interaction between PstCEP1 and PSTG_11208 inhibited the resistance enhancement by PSTG_11208. Furthermore, wheat apoplastic thaumatin-like protein 1 (TaTLP1) appeared recognizing Pst invasion by interacting with PSTG_11208 to initiate the downstream defense response by the pathogenesis-related protein TaPR1. Silencing TaTLP1 and TaPR1 separately or simultaneously reduced the HTSP resistance to Pst in XY6. Furthermore, we found that PstCEP1 targeted wheat ferredoxin 1 (TaFd1), a homologous protein of rice OsFd1. Silencing TaFd1 affected the stability of photosynthesis in wheat plants, resulting in chlorosis on the leaves and reducing the HTSP resistance. Our findings revealed the synergistic mechanism of effector proteins in the process of pathogen infection.