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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #401752
Research Project: Analysis of Genetic Factors that Increase Foodborne Pathogen Fitness, Virulence, and Antimicrobial Resistance Transfer, to Identify Interventions against Salmonella and Campylobacter in Food Animals

Location: Food Safety and Enteric Pathogens Research

Title: Swine microbiome changes in response to vaccination with Enterisol Salmonella T/C® and challenge with Salmonella enterica I 4,[5],12:i:-

Author
item BURCIAGO, SELMA - Oak Ridge Institute For Science And Education (ORISE)
item Trachsel, Julian
item LEITE, FERNANDO - Boehringer Ingelheim
item Bearson, Shawn

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Foodborne outbreaks caused by Salmonella enterica serovar I 4,[5],12:i:- have been reported worldwide, and illnesses are largely associated with the consumption of contaminated pork, implicating swine as an important reservoir of Salmonella I 4,[5],12:i:-. In the United States, Salmonella I 4,[5],12:i:- is a predominant Salmonella serovar in the swine population and is commonly isolated in clinical cases involving salmonellosis in swine. The aim of this study was to investigate the effects of Enterisol Salmonella T/C® vaccination in the context of a challenge with 2x109 CFU of Salmonella I 4,[5],12:i:- on the gut microbiota of pigs. Four-week-old weaned piglets were assigned to two treatment groups: (1) mock-vaccinated/challenged and (2) Enterisol Salmonella T/C® vaccinated/challenged (n=20 pigs/group). Fecal samples were collected the day prior to vaccination (-28 days post inoculation (challenge) with Salmonella I 4,[5],12:i:-; dpi), one day before challenge (-1 dpi), and subsequent days post challenge (2, 3, 7, 10, and 14 dpi). At 14 dpi, pigs were euthanized and cecal contents were collected. 16S rRNA gene sequencing of the V4 region was performed on DNA samples from fecal and cecal contents to characterize and compare the microbiomes of the treatment groups over time. Prior to vaccination (-28 dpi), a significant difference in bacterial community structure was not detected between the two pig groups. However, a significant difference in beta diversity (PERMANOVA) was observed between the two treatment groups four weeks after vaccination (-1 dpi) and at each timepoint post-challenge with Salmonella I 4,[5],12:i:- (2, 3, 7, 10, and 14 dpi). At 14 dpi, significant differences in the swine cecal microbiomes between the two treatment groups were not detected. Understanding alterations of the swine gut microbiome challenged with Salmonella I 4,[5],12:i:- and treated with Enterisol Salmonella T/C® may provide insight on mitigation strategies to control this serovar on the farm, thereby reducing salmonellosis in humans.