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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #401962

Research Project: Efficient and Effective Preservation and Management of Plant and Microbial Genetic Resource Collections

Location: Agricultural Genetic Resources Preservation Research

Title: Advances in plant cryobiology for shoot tip and dormant bud cryopreservation

Author
item Volk, Gayle

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/20/2023
Publication Date: 6/14/2023
Citation: Volk, G.M. 2023. Advances in plant cryobiology for shoot tip and dormant bud cryopreservation [abstract]. IV International Symposium on Plant Cryopreservation. p. 19.

Interpretive Summary: N/A

Technical Abstract: Plant shoot tip and dormant bud cryopreservation has become routine in many genebank and conservation programs. In part, this is due to the development of methods that are applicable across a wide range of diversity within genera as well as a highly skilled workforce that are able to implement difficult procedures. Remarkably, the underlying physiological, biophysical, and molecular mechanisms of successful cryopreservation procedures are not well understood. Collaborations among experts across a wide range of disciplines are revealing new insights that help explain how cryoprotectant solutions allow shoot tips to survive and recover from liquid nitrogen exposure. Although cryoprotectant solutions may not be needed for successful dormant bud cryopreservation, physiological and molecular processes allow these propagule types to survive in liquid nitrogen for decades. This presentation will include recent findings that have advanced our knowledge of the mechanistic aspects of shoot tip and dormant bud cryopreservation processes.