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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #402007
Research Project: Host-Pathogen Interactions Affecting Wheat and Barley

Location: Cereal Crops Improvement Research

Title: Population diversity of Xanthomonas translucens pv. translucens in North Dakota, Montana, and Idaho

Author
item VELASCO, DIEL - North Dakota State University
item BENZ, BROOKE - North Dakota State University
item NAVASAC, ABBEAH - North Dakota State University
item Schachterle, Jeffrey
item FRISKOP, ANDREW - North Dakota State University
item LIU, ZHAOHUI - North Dakota State University
item BALDWIN, THOMAS - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/27/2023
Publication Date: 3/2/2023
Citation: Velasco, D., Benz, B., Navasac, A., Schachterle, J.K., Friskop, A., Liu, Z., Baldwin, T. 2023. Population diversity of Xanthomonas translucens pv. translucens in North Dakota, Montana, and Idaho [abstract]. Bacterial Leaf Streak Conference. 2:7.

Interpretive Summary:

Technical Abstract: Bacterial leaf streak (BLS) disease is an increasing issue for barley growers in the top three barley production states in the United States, North Dakota, Idaho, and Montana. Resistance to BLS has yet to be established and management options have performed unreliably. This makes the threat of an outbreak remain a strong possibility. The pathogen affecting barley is primarily Xanthomonas translucens pv. translucens (Xtt). Recently, this pathogen was found to be more genetically diverse than previously thought, with three distinct clades carrying a diverse and variable array of effector genes. Yet, the population diversity in the three major barley-growing states is underexplored. To study the BLS pathogen diversity, we analyzed the genetic heterogeneity of 51 Xtt isolates collected from North Dakota (15), Montana (17) and Idaho (19) by comparing their whole-genomes sequenced through Oxford Nanopore Technology. Since genetic similarities of these isolates are very high (>95%), ANI was used for phylogenetic analysis. Type II, III and IV secretion systems, and predicted effectors, known to potentially play important roles on the pathogenicity, virulence, proliferation, and survival of Xanthomonas, were mapped across all sequenced genomes. We will report our preliminary findings on the diversity of Xtt across the three major barley-growing states. Understanding the genetic diversity of Xtt in these states aid in developing resistance in barley and management strategies. This isolate set can be used to study how Xtt is evolving in the barley production regions of the United States.