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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Animal Health Genomics » Research » Publications at this Location » Publication #402789
Research Project: Strategies to Control Respiratory Diseases of Cattle

Location: Animal Health Genomics

Title: Detection of Mannheimia haemolytica-specific IgG, IgM and IgA in sera and their relationship to respiratory disease in cattle

Author
item POONSUK, KORAKRIT - University Of Nebraska
item KORDIK, CARITA - University Of Nebraska
item HILLE, MATTHEW - University Of Nebraska
item CHENG, TING-YU - The Ohio State University
item CROSBY, WILLIAM - Mississippi State University
item WOOLUMS, AMELIA - Mississippi State University
item Clawson, Michael - Mike
item Chitko-Mckown, Carol
item BRODERSEN, BRUCE - University Of Nebraska
item LOY, JOHN - University Of Nebraska

Submitted to: Animals
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/25/2023
Publication Date: 5/3/2023
Citation: Poonsuk, K., Kordik, C., Hille, M., Cheng, T., Crosby, W.B., Woolums, A., Clawson, M.L., Chitko-McKown, C., Brodersen, B., Loy, J.D. 2023. Detection of Mannheimia haemolytica-specific IgG, IgM and IgA in sera and their relationship to respiratory disease in cattle. Animals. 13(9). Article 1531. https://doi.org/10.3390/ani13091531.
DOI: https://doi.org/10.3390/ani13091531

Interpretive Summary: Mannheimia haemolytica is a key contributor to bovine respiratory disease (BRD) in cattle. It is commonly found in calves and young cattle with pneumonia. Traditional visual assessments for BRD can be unreliable, leading to improper treatment and disease spread. In this study, three different ELISAs (enzyme-linked immunosorbent assays) were developed to detect different antibody types that cattle produce following exposure to, or infection by M. haemolytica. The three ELISAs have high diagnostic sensitivity and specificity. These ELISAs are important tools that can be used for surveillance of cattle immunity to M. haemolytica and prevention of BRD.

Technical Abstract: Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigen using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P >/= 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cut-off of sample to positive (S/P) ratio >/= 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P >/ = 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of M. haemolytica (p-value < 0.05). These data suggest that M. haemolytica ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.