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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #402894
Research Project: Improving Dairy Cow Feed Efficiency and Environmental Sustainability Using Genomics and Novel Technologies to Identify Physiological Contributions and Adaptations

Location: Animal Genomics and Improvement Laboratory

Title: Genome-wide acetylation modification of H3K27ac in bovine rumen cell following butyrate exposure

Author
item KANG, XIAOLONG - Ningxia University
item LI, CHENGLONG - Ningxia University
item LIU, SHULI - Westlake University
item Baldwin, Ransom - Randy
item Liu, Ge - George
item Li, Congjun - Cj

Submitted to: Biomolecules EISSN 2218-273X
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/13/2023
Publication Date: 7/16/2023
Citation: Kang, X., Li, C., Liu, S., Baldwin, R.L., Liu, G., Li, C.-J. 2023. Genome-wide acetylation modification of H3K27ac in bovine rumen cell following butyrate exposure. Biomolecules. 13(7):1137. https://doi.org/10.3390/biom13071137.
DOI: https://doi.org/10.3390/biom13071137

Interpretive Summary: Butyrate is an essential nutrient for cattle. It also has gene regulation activities. However, the mechanism and role of butyrate in gene regulation are still unclear. This study uses the ChIP-sequencing method to study the role of butyrate in the rumen epithelial primary cells. We found that butyrate induces genome-wide modification of histone acetylation. The alterations of histone acetylation regulate gene activities and change genomic actions. Identifying genome-wide acetylation modifications and expressed genes of butyrate in bovine rumen epithelial primary cells will expand the understanding of the biological role of butyrate and its effects on histone acetylation.

Technical Abstract: Butyrate contributes epigenetically to cellular function and rumen development in ruminant animals, which might be achieved by its genetic or epigenetic regulation of gene expression. To explore the role of butyrate on bovine rumen epithelial function and development, this study characterized genome-wide H3K27ac modification changes and super-enhancer profiles in rumen epithelial primary cell (REPC) induced with butyrate by ChIP-seq and analyzed its effects on the genes expression and functional pathways by integrating RNA-seq data. The results showed that the genome-wide acetylation modification (H3K27ac) was observed in the REPC with 94,675 and 48,688 peaks in the butyrate treatment and control group, respectively. Totally, 9,750 and 5,020 genes with increased modification (H3K27ac-gain) and decreased modification (H3K27ac-loss) were detected in the treatment group. The super-enhancers associated genes in the butyrate-induction group were involved in the AMPK signaling pathway, MAPK signaling pathway, and ECM-receptor interaction. Finally, the up-regulated genes (PLCG1, CLEC3B, IGSF23, OTOP3, ADTRP) with H3K27ac gain modification by butyrate were involved in cholesterol metabolism, lysosome, cell adhesion molecules, and PI3K-Akt signaling pathway. Butyrate treatment has the role of genome-wide H3K27ac acetylation on bovine REPC and affects the changes in gene expression. The effect of butyrate on gene expression correlates with the acetylation of the H3K27ac level. Identifying genome-wide acetylation modifications and expressed genes of butyrate in bovine REPC cells will expand the understanding of the biological role of butyrate and its acetylation.