In Vitro Effects of Myo-Inositol Supplementation on Adult Human Neural Progenitor Cells

Authors: MYKYTEN, KATHRYN - Jean Mayer Human Nutrition Research Center On Aging At Tufts University; Fisher, Derek; BIEDERER, THOMAS - Jean Mayer Human Nutrition Research Center On Aging At Tufts University; ZHENG, TONG - Jean Mayer Human Nutrition Research Center On Aging At Tufts University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/3/2023
Publication Date: 7/22/2023
Citation: Mykyten, K., Fisher, D.R., Biederer, T., Zheng, T. 2023. In Vitro Effects of Myo-Inositol Supplementation on Adult Human Neural Progenitor Cells. [Abstract]. 7(Suppl 1):101233. https://doi.org/10.1016/j.cdnut.2023.101233.
DOI: https://doi.org/10.1016/j.cdnut.2023.101233

Interpretive Summary:

Technical Abstract: Objectives: Hippocampal neural progenitor cells are responsible for the generation of new neurons in the hippocampus, a brain region heavily involved in memory and learning. Impairments to neurogenesis have been tied to age- and disease- related cognitive decline and linked to oxidative stress states. When supplemented through diet, myo-inositol (M-I), the primary inositol found in brain tissue, crosses the blood brain barrier and has shown modulatory effects on brain structure and function. This study investigated the possible protective effects of M-I on hippocampal adult human neural progenitor cell (AHNP) proliferation and survival in vitro with and without an oxidative stress challenge. Methods: AHNPs derived from the healthy human hippocampi were maintained in N2 medium with 5% fetal bovine serum, bovine pituitary extract, growth factors, and treated with myo-inositol for 7 days (1-2 mM) before inducing oxidative stress. For stressed experimental groups, cells were incubated for 24 hours with 200 µM hydrogen peroxide-containing media, while non-stressed groups remained under control media with no hydrogen peroxide. Cell survival was measured using Trypan Blue exclusion method, and proliferation rates were measured using the ethynyl-deoxyuridine (EdU) assay and fluorescence microscopy. Quantified data was compared among experimental groups. Results: Our data showed that M-I did not have significant effects on the survival and proliferation rate of the non-stressed AHNPs. However, myo-inositol treatments reversed decreases in both survival (both 1 mM and 2 mM treatments) and proliferation rate (2 mM) of AHNPs following stress induced by H2O2. Conclusions: This study suggests that myo-inositol may be beneficial in fortifying hippocampal neural progenitor cells against oxidative stress, suggesting a neuroprotective role in aging neurogenesis. Future study is required to elucidate the mechanism underlying these observations.