Registration of Golden Promise/Otis barley recombinant inbred line mapping population

Authors: Bregitzer, Paul; Ajayi, Oyeyemi; Esvelt Klos, Kathy; Gao, Dongying; Mahalingam, Ramamurthy

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/29/2023
Publication Date: 10/29/2023
Citation: Bregitzer, P.P., Ajayi, O.O., Esvelt Klos, K.L., Gao, D., Mahalingam, R. 2023. Registration of Golden Promise/Otis barley recombinant inbred line mapping population. Journal of Plant Registrations. 18:197-203. https://doi.org/10.1002/plr2.20337.
DOI: https://doi.org/10.1002/plr2.20337

Interpretive Summary: Golden Promise is heritage malting barley variety from the UK well known among the malting and brewing industry for its flavor properties. Otis is a hertiage feed barley variety suitable for the drylands. Otis is tolerant to abiotic stresses like drought and heat while GP is sensitive. A population was developed from a cross between Golden Promise and Otis. This population consisiting of 192 individuals has been selfed for more than five generations and hence is very stable. It provides a valuable resource for genetic mapping of complex traits like drought and heat tolerance. Each of these lines have been genotyped using the Barley 50,000 genechip arrays. This population can hence be further used to explore the genetic basis of these abiotic stresses. The seeds of this population will be available through the germplasm resource center for other researchers. Furthermore, the genetic information for the population will be freely accessible through the Triticeae tool box. These resources will aid in gaining understanding the genetic basis of complex traits and provide tools for breeding climate resilient crops with improved end quality traits.

Technical Abstract: A recombinant inbred line mapping population was developed from a cross between the heritage malting barley (Hordeum vulgare Subsp. vulgare) variety Golden Promise and Otis, a heritage feed barley variety developed in the 1960s suitable for the high dry plains. The Gp/Otis RIL population consists of 192 F5:7 individuals. The parental lines exhibited significant differences in several agronomic traits including height, heading date, yield/plant, and straw strength. Transgressive segregation in the RILs was evidenced by the broad range of values observed for these agronomic traits. The RIL population was genotyped using the Illumina 50k iSelect SNP arrays. Of the 10,810 polymorphic markers that were identified between the two parents, 4,617 markers were used for generating a genetic map that spanned 4,566 cM with an average distance of 1cM between markers. This population has been used for identifying QTLs associated with drought tolerance. Otis has been observed to be tolerant to heat and a combination of heat and drought stress. This population can hence be further used to explore the genetic basis of these abiotic stresses. The fact that GP was developed in the UK while Otis was bred in the USA, this population may be useful in identifying other novel traits of interest.