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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #404739
Research Project: Introgression of Disease Resistance and Tuber Quality Traits from Wild Species Relatives into Diploid Cultivated Potato

Location: Vegetable Crops Research

Title: QTL-seq analysis and linkage mapping reveal genomic regions contributing to the epicotyl length trait in adzuki bean (Vigna angularis)

Author
item KACHAPILA, MODESTER - Obihiro University Of Agriculture And Veterinary Medicine
item HORIUCHI, YUKI - Tokachi Agricultural Experiment Station
item NAGASAWA, HIDETAKA - Tokachi Agricultural Experiment Station
item MICHIHATA, NOE - Obihiro University Of Agriculture And Veterinary Medicine
item YOSHIDA, TORU - Obihiro University Of Agriculture And Veterinary Medicine
item KATO, YUTA - Obihiro University Of Agriculture And Veterinary Medicine
item Bethke, Paul
item KATO, KIYOAKI - Obihiro University Of Agriculture And Veterinary Medicine
item MORI, MASAHIKO - Obihiro University Of Agriculture And Veterinary Medicine

Submitted to: Agriculture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/22/2023
Publication Date: 6/26/2023
Citation: Kachapila, M., Horiuchi, Y., Nagasawa, H., Michihata, N., Yoshida, T., Kato, Y., Bethke, P.C., Kato, K., Mori, M. 2023. QTL-seq analysis and linkage mapping reveal genomic regions contributing to the epicotyl length trait in adzuki bean (Vigna angularis). Agriculture. https://doi.org/10.3390/agriculture13071305.
DOI: https://doi.org/10.3390/agriculture13071305

Interpretive Summary: Mechanical weeding and harvest of adzuki beans would cause less damage to the crop if the length of the stem below the leaves and bean pods was longer. The length of this part of the stem is genetically controlled, but the precise genes involved are not known. In this research, four locations within the adzuki bean DNA that contribute to greater stem length were identified and two of these were confirmed using a second set of adzuki bean plants. Further analysis narrowed down the size one of these regions, and identified molecular markers associated with it. These findings will assist breeders of adzuki bean to develop varieties that are better suited for mechanical weeding and harvesting. This, in turn, will increase the efficiency of adzuki bean production, benefiting growers and consumers of adzuki beans.

Technical Abstract: Increasing the epicotyl length (ECL) of adzuki bean cultivars makes them more suitable for mechanical weeding and harvesting. To explore the genetic control of epicotyl length, and to identify molecular markers that could facilitate breeding for increased ECL, a mapping population was developed from a cross between Toiku 161 (long epicotyls) and Chihayahime (ordinary epicotyls). In this study, four quantitative trait loci (QTLs) for ECL were identified, one each on chromosomes 2, 7, 10 and 11 by QTL-seq analysis. InDel-based mapping confirmed the major QTLs on chromosomes 7, qECL7.1, and 10, qECL10.1. Substitution mapping using InDel, CAPS, dCAPS, and SNP markers narrowed the chromosomal location of qECL7.1 to a 418 kb region flanked by DNA markers TC37_10,211,134 bp and TC40_10,628,880 bp. A total of 35 predicted genes were within the qECL7.1 region. The ECL QTLs and associated candidate genes identified here will contribute towards marker-assisted selection of desirable long ECL genotypes that allow for increased mechanization and more efficient adzuki bean production.